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首页> 外文期刊>Parasitology >Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes?
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Redundancy and recombination in the Echinococcus AgB multigene family: is there any similarity with protozoan contingency genes?

机译：棘球Ag虫AgB多基因家族中的冗余和重组：与原生动物偶然性基因有相似之处吗？

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摘要

Numerous genetic variants of the Echinococcus antigen B (AgB) are encountered within a single metacestode. This could be a reflection of gene redundancy or the result of a somatic hypermutation process. We evaluate the complexity of the AgB multigene family by characterizing the upstream promoter regions of the 4 already known genes (EgAgB1-EgAgB4) and evaluating their redundancy in the genome of 3 Echinococcus species (E. granulosus, E. ortleppi and E. multilocularis) using PCR-based approaches. We have ascertained that the number of AgB gene copies is quite variable, both within and between species. The most repetitive gene seems to be AgB3, of which there are more than 110 copies in E. ortleppi. For E. granulosus, we have cloned and characterized 10 distinct upstream promoter regions of AgB3 from a single metacestode. Our sequences suggest that AgB1 and AgB3 are involved in gene conversion. These results are discussed in light of the role of gene redundancy and recombination in parasite evasion mechanisms of host immunity, which at present are known for protozoan organisms, but virtually unknown for multicellular parasites.
机译：棘球E球菌抗原B（AgB）的许多遗传变异体都在一个单个的大肠内。这可能反映了基因冗余或体细胞超突变过程的结果。我们通过表征4个已知基因（EgAgB1-EgAgB4）的上游启动子区域并评估其在3种棘球oc种（E. granulosus，E。ortleppi和E. multilocularis）的基因组中的冗余来评估AgB多基因家族的复杂性。使用基于PCR的方法。我们已经确定，在物种内部和物种之间，AgB基因拷贝的数量是相当可变的。最重复的基因似乎是AgB3，其中在E. ortleppi中有110多个拷贝。对于E. granulosus，我们已经从一个单一的胞囊中克隆并鉴定了10个不同的AgB3上游启动子区域。我们的序列表明AgB1和AgB3参与基因转换。鉴于基因冗余和重组在宿主免疫的寄生虫逃逸机制中的作用，讨论了这些结果，目前对于原生动物而言这是已知的，但对于多细胞寄生虫却几乎未知。

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