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首页> 外文期刊>Parasitology >Evaluation of an indirect fluorescence immunoassay for the detection of serum antibodies against Babesia divergens in humans.
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Evaluation of an indirect fluorescence immunoassay for the detection of serum antibodies against Babesia divergens in humans.

机译：评估间接荧光免疫测定法以检测人类抗巴氏杆菌多样性的血清抗体。

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摘要

Since an indirect fluorescence immunoassay (IFA) for the detection of specific antibodies against Babesia divergens in human sera is not commercially available, an in-house prepared B. divergens IFA for the examination of bovine sera was established for serological studies in humans. To determine whether the described IFA is appropriate for such studies, 2 B. divergens antigens (of human or bovine origin) were tested using serum samples obtained from febrile human patients with a history of 'tick bite'. Sera from other species of animals infected with B. divergens, Babesia EU1, B. microti or B. canis were also included for comparative purposes. All serum samples were also tested using a commercially available IFA for the detection of antibodies against B. microti, and the results compared with those obtained using blood smear and molecular techniques. This study showed that the evaluation and standardization of a B. divergens IFA for testing human sera is critical and that different B. divergens antigens provide different end-point titres of antibodies, leading to false negative or positive results. Serological cross-reactivity between B. divergens and Babesia EU1 needs to be taken into account when interpreting IFA results.
机译：由于用于检测人血清中抗巴氏杆菌多样性的特异性抗体的间接荧光免疫测定（IFA）尚无商业销售，因此建立了用于检查牛血清的内部制备的B. divergens IFA，用于人体血清学研究。为了确定所描述的IFA是否适合于此类研究，使用得自具有“ history咬”病史的发热人类患者的血清样品测试了2种（人类或牛源的）B。divergens抗原。出于比较目的，也包括来自其他物种的感染了博登芽孢杆菌，Babesia EU1，B。microti或B. canis的动物的血清。还使用市售IFA对所有血清样品进行了测试，以检测针对微小芽孢杆菌的抗体，并将结果与​​使用血液涂片和分子技术获得的结果进行了比较。这项研究表明，曲霉芽孢杆菌IFA的测试和评估对于评估人血清至关重要，而且不同的芽孢杆菌多样性抗原提供的抗体终点滴度也不同，从而导致假阴性或阳性结果。解释IFA结果时，应考虑B. divergens和巴贝斯虫EU1之间的血清学交叉反应。

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