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首页> 外文期刊>Parasitology >A role for antimicrobial peptides in intestinal microsporidiosis.
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A role for antimicrobial peptides in intestinal microsporidiosis.

机译：抗菌肽在肠道微孢子虫病中的作用。

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SUMMARY: Clinical isolates from 3 microsporidia species, Encephalitozoon intestinalis and Encephalitozoon hellem, and the insect parasite Anncaliia (Brachiola, Nosema) algerae, were used in spore germination and enterocyte-like (C2Bbe1) cell infection assays to determine the effect of a panel of antimicrobial peptides. Spores were incubated with lactoferrin (Lf), lysozyme (Lz), and human beta defensin 2 (HBD2), human alpha defensin 5 (HD5), and human alpha defensin 1 (HNP1), alone and in combination with Lz, prior to germination. Of the Encephalitozoon species only E. hellem spore germination was inhibited by HNP1, while A. algerae spore germination was inhibited by Lf, HBD2, HD5 and HNP1, although HBD2 and HD5 inhibition required the presence of Lz. The effects of HBD2 and HD5 on microsporidia enterocyte infection paralleled their effects on spore germination. Lysozyme alone only inhibited infection with A. algerae, while Lf inhibited infection by E. intestinalis and A. algerae. HNP1 significantly reduced enterocyte infection by all 3 parasite species and a combination of Lf, Lz and HNP1 caused a further reduced infection with A. algerae. These data suggest that intestinal antimicrobial peptides contribute to the defence of the intestine against infection by luminal microsporidia spores and may partially determine which parasite species infects the intestine.
机译：摘要：来自3种小孢子虫的临床分离株，即小肠脑虫和小脑乙虫，以及阿尔及利亚昆虫寄生虫Anncaliia（Brachiola，Nosema），被用于孢子萌发和肠样细胞（C2Bbe1）细胞感染测定，以确定一组检测结果。抗菌肽。萌发前，将孢子与乳铁蛋白（Lf），溶菌酶（Lz）和人β防御素2（HBD2），人α防御素5（HD5）和人α防御素1（HNP1）一起孵育，然后使其萌发。 。在Encephalitozoon物种中，只有HNP1抑制了大肠埃希菌的孢子萌发，而Lf，HBD2，HD5和HNP1抑制了阿尔及利亚芽孢的孢子萌发，尽管HBD2和HD5的抑制作用需要Lz的存在。 HBD2和HD5对小孢子虫肠细胞感染的作用与其对孢子萌发的作用平行。单独的溶菌酶仅抑制阿尔及利亚杆菌的感染，而Lf抑制肠杆菌和阿尔及利亚杆菌的感染。 HNP1显着降低了所有3种寄生虫物种对肠细胞的感染，Lf，Lz和HNP1的组合进一步降低了阿尔及利亚曲霉的感染。这些数据表明，肠道抗菌肽有助于肠道防御腔小孢子虫孢子的感染，并可能部分确定哪种寄生虫会感染肠道。

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