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首页> 外文期刊>Parasitology >Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii.
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Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii.

机译:迈向弓形虫分子检测和鉴定的综合方法。

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摘要

The development of simple, sensitive and rapid methods for the detection and identification of Toxoplasma gondii is important for the diagnosis and epidemiological studies of the zoonotic disease toxoplasmosis. In the past 2 decades, molecular methods based on a variety of genetic markers have been developed, each with its advantages and limitations. The application of these methods has generated invaluable information to enhance our understanding of the epidemiology, population genetics and phylogeny of T. gondii. However, since most studies focused solely on the detection but not genetic characterization of T. gondii, the information obtained was limited. In this review, we discuss some widely used molecular methods and propose an integrated approach for the detection and identification of T. gondii, in order to generate maximum information for epidemiological, population and phylogenetic studies of this key pathogen.
机译:开发简单,灵敏和快速的方法来检测和鉴定弓形虫对人畜共患病弓形虫病的诊断和流行病学研究很重要。在过去的20年中,已经开发了基于多种遗传标记的分子方法,每种方法都有其优点和局限性。这些方法的应用已经产生了宝贵的信息,以增进我们对弓形虫的流行病学,种群遗传学和系统发育的了解。但是,由于大多数研究仅侧重于弓形虫的检测而不是遗传特征,因此获得的信息有限。在这篇综述中,我们讨论了一些广泛使用的分子方法,并提出了一种检测和鉴定弓形虫的综合方法,以便为这种关键病原体的流行病学,种群和系统发育研究提供最大的信息。

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