...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Trypanosoma melophagium from the sheep ked Melophagus ovinus on the island of St Kilda.
【24h】

Trypanosoma melophagium from the sheep ked Melophagus ovinus on the island of St Kilda.

机译：从绵羊的锥虫锥虫在圣基尔达海岛上在Ked Melophagus ovinus绵羊上。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

SUMMARYThe sheep ked has been largely eradicated in the UK but persists in the feral Soay sheep of St Kilda in the Outer Hebrides. Sheep keds transmit Trypanosoma melophagium, but parasitaemias are typically cryptic and this trypanosome has not been recorded in the St Kilda sheep. Trypanosomes were detected by PCR in preserved keds and were also found in gut smears from live keds; one infected gut was used to establish the trypanosome in vitro. Examination of the morphology of bloodstream forms from culture confirmed its identity as T. melophagium. Most keds were found to harbour the trypanosome, particularly those collected from lambs. DNA was extracted from preserved keds and from trypanosomes grown in vitro. Sequence analysis of the small subunit ribosomal RNA (SSU rRNA) gene and the spliced leader transcript showed the T. melophagium sequences to be very similar to those from T. theileri. A partial sequence of the ked SSU rRNA gene was also obtained. The close genetic relationship of T. melophagium and T. theileri suggests that T. melophagium represents a lineage of T. theileri that adapted to transmission by sheep keds and hence became a specific parasite of sheep.
机译：总结在英国，基德的羊群已基本消灭，但在外赫布里底群岛的圣基尔达的野生苏伊羊群中仍然存在。羊科动物传播梅毒锥虫，但寄生虫病通常是隐性的，圣基尔达羊中尚未记录到这种锥虫。锥虫小体通过PCR检测到了保存的小动物中的锥虫，也发现于活小动物的肠涂片中。用一只感染的肠道体外建立锥虫体。从培养物中检查血流形式的形态证实了其身份为T. melophagium。发现大多数科德人都携带锥虫，特别是从羔羊身上收集的锥虫。从保存的keds和体外生长的锥虫中提取DNA。对小亚基核糖体RNA（SSU rRNA）基因和剪接的前导转录本的序列分析表明，T。melophagium序列与T. theileri序列非常相似。还获得了ked SSU rRNA基因的部分序列。 T. melophagium和T. theileri的密切遗传关系表明，T。melophagium代表了T. theileri的世系，适应于羊科动物的传播，因此成为绵羊的特定寄生虫。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号