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首页> 外文期刊>Parasitology >Isolation of Trypanosoma caninum in domestic dogs in Rio de Janeiro, Brazil.
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Isolation of Trypanosoma caninum in domestic dogs in Rio de Janeiro, Brazil.

机译：巴西里约热内卢家犬的锥虫锥虫的分离。

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SUMMARY: The domestic dog's involvement with different members of the Trypanosomatidae family has been the focus of several studies due to this animal's close proximity to man. Recently this animal has been infected by a new Trypanosoma species (T. caninum), described in Rio de Janeiro and 19 similar isolates were later obtained. The objective of this study was to identify these isolates. All samples were isolated from intact skin cultures and analysed morphologically, by biochemical isoenzyme electrophoresis assays and by several molecular PCR assays. Additionally, anti-Leishmania sp. antibodies were assessed using the indirect Immunofluorescence Antibody Test (IFAT) in all animals. The methodologies employed to identify the isolates, including partial nucleotide sequences of 18S rRNA gene, indicated patterns identical to T. caninum and patterns different from the other species, including T. cruzi and T. rangeli samples. A phylogenetic tree constructed with the partial 18S ribosomal sequence shows that T. caninum is clustered with T. pestanai. Ten (52.6%) animals presented anti-Leishmania sp. antibodies with titres varying from 1:40 to 1:320. Thus, the hypothesis that this protozoan has disseminated among the dogs in Rio de Janeiro must be considered. The importance of a correct diagnosis in those animals and the possible consequences in the areas where visceral leishmaniasis is found are discussed here.
机译：摘要：由于这种动物与人的亲密关系，家犬对锥虫科不同成员的参与一直是许多研究的重点。最近，该动物已被里约热内卢描述的一种新的锥虫（T. caninum）感染，后来获得了19种类似的分离株。本研究的目的是鉴定这些分离株。从完整的皮肤培养物中分离出所有样品，并通过生化同工酶电泳测定法和几种分子PCR测定法进行形态分析。此外，抗利什曼原虫。使用间接免疫荧光抗体测试（IFAT）在所有动物中评估抗体。用于鉴定分离物的方法学，包括18S rRNA基因的部分核苷酸序列，显示了与犬科螺旋杆菌相同的模式以及与其他物种（包括克鲁斯锥虫和兰吉拉锥虫）样品不同的模式。用部分18S核糖体序列构建的系统发育树显示，犬热疫病菌与鼠疫热病菌聚在一起。十只（52.6％）动物表现出抗利什曼原虫菌种。滴度从1:40到1：320的抗体。因此，必须考虑这种原生动物已经在里约热内卢的狗中传播的假说。这里讨论正确诊断这些动物的重要性以及发现内脏利什曼病的可能后果。

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