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首页> 外文期刊>Parasitology >Involvement of host cell heparan sulfate proteoglycan in Trypanosoma cruzi amastigote attachment and invasion.
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Involvement of host cell heparan sulfate proteoglycan in Trypanosoma cruzi amastigote attachment and invasion.

机译：宿主细胞硫酸乙酰肝素蛋白聚糖参与克氏锥虫鞭毛附着和侵袭。

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摘要

Cell surface glycosaminoglycans (GAGs) play an important role in the attachment and invasion process of a variety of intracellular pathogens. We have previously demonstrated that heparan sulfate proteoglycans (HSPG) mediate the invasion of trypomastigote forms of Trypanosoma cruzi in cardiomyocytes. Herein, we analysed whether GAGs are also implicated in amastigote invasion. Competition assays with soluble GAGs revealed that treatment of T. cruzi amastigotes with heparin and heparan sulfate leads to a reduction in the infection ratio, achieving 82% and 65% inhibition of invasion, respectively. Other sulfated GAGs, such as chondroitin sulfate, dermatan sulfate and keratan sulfate, had no effect on the invasion process. In addition, a significant decrease in infection occurred after interaction of amastigotes with GAG-deficient Chinese Hamster Ovary (CHO) cells, decreasing from 20% and 28% in wild-type CHO cells to 5% and 9% in the mutant cells after 2 h and 4 h of infection, respectively. These findings suggest that amastigote invasion also involves host cell surface heparan sulfate proteoglycans. The knowledge of the mechanism triggered by heparan sulfate-binding T. cruzi proteins may provide new potential candidates for Chagas disease therapy.
机译：细胞表面糖胺聚糖（GAG）在多种细胞内病原体的附着和侵袭过程中起重要作用。我们以前已经证明，硫酸乙酰肝素蛋白聚糖（HSPG）介导了心肌细胞中锥虫锥虫锥虫形式的侵袭。在本文中，我们分析了GAGs是否也与鞭毛体入侵有关。用可溶性GAG进行竞争分析表明，用肝素和硫酸乙酰肝素治疗克氏锥虫的感染率降低，分别实现了82％和65％的抑制入侵。其他硫酸化的GAG，例如硫酸软骨素，硫酸皮肤素和硫酸角质素，对入侵过程没有影响。此外，变形虫与GAG缺陷型中国仓鼠卵巢（CHO）细胞相互作用后，感染发生率显着降低，从2型后野生型CHO细胞的20％和28％降至突变型细胞的5％和9％分别感染h和4 h。这些发现表明，鞭毛体侵入也涉及宿主细胞表面硫酸乙酰肝素蛋白聚糖。硫酸乙酰肝素结合克鲁维酵母蛋白触发的机制的知识可能为南美锥虫病治疗提供新的潜在候选人。

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