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首页> 外文期刊>Parasitology >Involvement of sulfated glycosaminoglyeans on the development and attachment of Trypanosoma cruzi to the luminal midgut surface in the vector, Rhodnius prolixus
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Involvement of sulfated glycosaminoglyeans on the development and attachment of Trypanosoma cruzi to the luminal midgut surface in the vector, Rhodnius prolixus

机译：硫酸糖胺聚糖参与载体克氏罗得氏菌（Rhodnius prolixus）克鲁氏锥虫的发育和附着于腔中肠表面的过程

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摘要

In the present study, we investigated the involvement of sulfated glycosaminoglyeans in both the in vivo development and adhesion of T. cruzi epimastigotes to the luminal surface of the digestive tract of the insect vector, Rhodnius prolixus. Pre-incubation of T. cruzi, Dm 28c epimastigotes with heparin, chondroitin 4-sulfate, chondroitin 6-sulfate or protamine chloridrate inhibited in vitro attachment of parasites to the insect midgut. Enzymatic removal of heparan sulfate moieties by heparinase I or of chondroitin sulfate moieties by chondroitinase AC from the insect posterior midgut abolished epimastigote attachment in vitro. These treatments also reduced the labelling of anionic sites exposed at the luminal surface of the perimicrovillar membranes in the triatomine midgut epithelial cells. Inclusion of chondroitin 4-sulfate or chondroitin 6-sulfate and to a lesser extent, heparin, in the T. cruzi-infected bloodmeal inhibited the establishment of parasites in R. prolixus. These observations indicate that sulfated glycosaminoglyeans are one of the determinants for both adhesion of the T. cruzi epimastigotes to the posterior midgut epithelial cells of the triatomine and the parasite infection in the insect vector, R. prolixus.
机译：在本研究中，我们调查了硫酸糖胺聚糖在体内的发育和克鲁维氏锥虫的附着和在昆虫载体消化道（Rhodnius prolixus）消化道腔表面的粘附。用肝素，4-硫酸软骨素，6-硫酸软骨素或盐酸鱼精蛋白预孵育克鲁维氏梭菌，Dm 28c副鞭毛动物会抑制寄生虫在昆虫中肠的体外附着。肝素酶I酶促去除硫酸乙酰肝素部分或软骨素酶AC去除昆虫后中肠的硫酸软骨素部分，在体外消除了淫羊ote的附着。这些处理还减少了在三atomine中肠上皮细胞中微孔膜周围腔表面暴露的阴离子位点的标记。在克氏锥虫感染的血粉中加入4-硫酸软骨素或6-硫酸软骨素以及较小程度的肝素会抑制螺旋藻中寄生虫的建立。这些观察结果表明，硫酸化的糖胺聚糖是克鲁格氏锥虫近鞭毛象附着于三atomine的后肠中上皮细胞和昆虫载体R. prolixus中的寄生虫感染的决定因素之一。

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