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首页> 外文期刊>Parasitology >Vector of Trypanosoma copemani identified as Ixodes sp.
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Vector of Trypanosoma copemani identified as Ixodes sp.

机译：被鉴定为Ixodes sp。的锥虫锥虫载体。

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A total of 41 ticks were collected from 15 quokkas on Bald Island and 2 ticks from a Gilbert's potoroo from Two Peoples Bay. Three species of Ixodid ticks Ixodes australiensis, Ixodes hirsti and Ixodes myrmecobii were identified on the quokkas known to have a high prevalence of Trypanosoma copemani. Tick faeces from ticks isolated from 8 individual quokkas and a Gilbert's potoroo were examined with one identified as positive for trypanosomes. Faecal examination revealed trypanosomes similar to in vitro life-cycle stages of T. copemani. In total 12 ticks were dissected and trypanosomes found in sections of their midgut and haemolymph, 49 and 117 days after collection. Tick faeces, salivary glands and midguts from I. australiensis were screened using an 18S rRNA PCR with amplification seen only from the midguts. Sequencing showed 100% homology to T. copemani (genotype A) and 99-9% homology to the wombat (All) isolate of T. copemani. Trypanosomes were only detected in I. australiensis as neither I. hirsti nor I. myrmecobii survived the initial 30-day storage conditions. We therefore identify a vector for T. copemani as I. australiensis and, given the detection of trypanosomes in the faeces, suggest that transmission is via the faecal-oral route.
机译：从秃头岛上的15只短尾羚收集了41条s虫，并从“两个人海湾”的吉尔伯特坑中收集了2条tick虫。在已知具有高锥虫锥虫流行性的上鉴定出三种x虱，澳洲us，hirsti和myrmecobii。检查了从8个独立种群中分离出来的bert的粪便和吉尔伯特猫的粪便，发现其中之一对锥虫呈阳性。粪便检查显示锥虫类似于拟南芥的体外生命周期阶段。收集后第49天和117天，共解剖了12个tick虫，并在其中肠和血淋巴中发现了锥虫。使用18S rRNA PCR筛选来自澳大利西亚猪li的粪便，唾液腺和中肠，仅从中肠进行扩增。测序显示与Co.copemani（基因型A）有100％的同源性，与T.copemani的袋熊（所有）分离物有99-9％的同源性。锥虫仅在澳大利亚伊斯拉虫中被检测到，因为伊斯氏伊斯提尔氏菌和myrmecobii菌都没有在最初的30天储存条件下存活。因此，我们确定了T. copemani的载体为I. australiensis，并且鉴于在粪便中检测到锥虫，建议通过粪便-口腔途径进行传播。

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