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首页> 外文期刊>Parasitology >Genetic characterization of members of the genus Contracaecum (Nematoda: Anisakidae) from fish-eating birds from west-central Florida, USA, with evidence of new species.
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Genetic characterization of members of the genus Contracaecum (Nematoda: Anisakidae) from fish-eating birds from west-central Florida, USA, with evidence of new species.

机译：来自美国佛罗里达州中西部食鱼鸟类的对虾属（线虫：Anisakidae）成员的遗传特征，并有新物种的证据。

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摘要

Specimens of Contracaecum spp. from Phalacrocorax auritus and Pelecanus occidentalis from Florida were characterized by sequencing of the small subunit of the mitochondrial ribosomal RNA gene (rrnS) and by PCR-based RFLP analysis of the same gene and of the internal transcribed spacers (ITS) of nuclear ribosomal DNA. Analyses of the rrnS sequence data using the MP and UPGMA approaches yielded trees with similar topologies, delineating 3 main clusters. Specimens from Ph. auritus, morphologically assigned to C. rudolphii (s.l.), were part of the cluster comprising also the other 2 species of the C. rudolphii complex (A and B), but representing a genetically distinct group, potentially corresponding to a distinct lineage within the complex, provisionally named as C. rudolphii C. The second cluster comprised 5 individuals from P. occidentalis, which formed a genetically relatively homogeneous group. The rrnS data indicate that these specimens (indicated as Contracaecum sp. 1) are clearly genetically different from the morphologically most closely related species, i.e. C. rudolphii (s.l.) and C. microcephalum, and could represent a new species. The third cluster comprised 7 specimens from P. occidentalis morphologically assigned to C. multipapillatum (s.l.). These were shown to be genetically homogeneous and related to but quite distinct from C. multipapillatum from Greece, although additional studies are needed to assess their status. PCR-RFLP based markers for the quick identification of these taxa are provided.
机译：tra菜的标本。通过对线粒体核糖体RNA基因（rrnS）的小亚基进行测序，并对同一基因和核糖体DNA的内部转录间隔子（ITS）进行基于PCR的RFLP分析，对来自佛罗里达州的Ph螺和西洋国ele的特征进行了测序。使用MP和UPGMA方法对rrnS序列数据进行分析，得出具有相似拓扑的树，描绘了3个主要簇。从形态学上被分配给鲁道夫隐孢子虫（S1）的金耳科的标本是该集群的一部分，还包括鲁道夫隐孢子虫复合体的其他2种（A和B），但代表了遗传上不同的组，可能对应于复合体中的不同血统，临时命名为鲁道夫梭菌C。第二个簇包括来自西方疫霉菌的5个个体，形成了遗传上相对同质的群体。 rrnS数据表明，这些标本（指示为Contracaecum sp。1）在遗传上明显不同于形态上最密切相关的物种，即rudolphii（s.l.）和C. microcephalum，并且可以代表一个新物种。第三组包括7个来自西方疟原虫的标本，在形态上分配给多乳头假丝酵母（s.l.）。这些被证明是遗传上同质的，与希腊的多毛乳头瘤相关但很不同，尽管还需要进一步的研究来评估它们的状态。提供了基于PCR-RFLP的标记，可快速识别这些分类单元。

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