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首页> 外文期刊>Parasitology >Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential.
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Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential.

机译：通过PCR直接鉴定粪便中的囊胚芽孢杆菌和人畜共患病潜力的证据。

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In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.
机译：体外繁殖后再进行PCR，并利用一种能够直接检测粪便中胚芽孢杆菌的基于PCR的方法，来检测澳大利亚各种寄主（包括灵长类动物及其来自珀斯动物园的处理者）的胚芽孢杆菌。此外，还对来自泰国本地特有社区的狗和人的囊胚分离株进行了遗传学鉴定。直接从粪便中进行基于PCR的检测与检测体外培养的囊肿相比，具有更高的灵敏度。此外，对在PCR之前使用体外技术扩增的囊胚分离物的系统发育分析表明，该方法比5亚型更有利于Blastocystis亚型5的优先扩增。该研究首次提供了基于分子的证据，支持狗中Blastocystis的人畜共患病潜力，负鼠和灵长类动物处于自然环境中。

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