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首页> 外文期刊>Parasitology >Trypanosoma cruzi heparin-binding proteins mediate the adherence of epimastigotes to the midgut epithelial cells of Rhodnius prolixus
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Trypanosoma cruzi heparin-binding proteins mediate the adherence of epimastigotes to the midgut epithelial cells of Rhodnius prolixus

机译：克鲁氏锥虫肝素结合蛋白介导表鞭鞭毛虫粘附于罗氏梭菌的中肠上皮细胞

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摘要

Heparin-binding proteins (HBPs) have been demonstrated in both infective forms of Trypanosoma cruzi and are involved in the recognition and invasion of mammalian cells. In this study, we evaluated the potential biological function of these proteins during the parasite-vector interaction. HBPs, with molecular masses of 65·8 kDa and 59 kDa, were isolated from epimastigotes by heparin affinity chromatography and identified by biotin-conjugated sulfated glycosaminoglycans (GAGs). Surface plasmon resonance biosensor analysis demonstrated stable receptor-ligand binding based on the association and dissociation values. Pre-incubation of epimastigotes with GAGs led to an inhibition of parasite binding to immobilized heparin. Competition assays were performed to evaluate the role of the HBP-GAG interaction in the recognition and adhesion of epimastigotes to midgut epithelial cells of Rhodnius prolixus. Epithelial cells pre-incubated with HBPs yielded a 3·8-fold inhibition in the adhesion of epimastigotes. The pre-treatment of epimastigotes with heparin, heparan sulfate and chondroitin sulfate significantly inhibited parasite adhesion to midgut epithelial cells, which was confirmed by scanning electron microscopy. We provide evidence that heparin-binding proteins are found on the surface of T. cruzi epimastigotes and demonstrate their key role in the recognition of sulfated GAGs on the surface of midgut epithelial cells of the insect vector.
机译：肝素结合蛋白（HBP）已在克氏锥虫的两种感染形式中得到证实，并参与了哺乳动物细胞的识别和侵袭。在这项研究中，我们评估了这些蛋白质在寄生虫-载体相互作用过程中的潜在生物学功能。通过肝素亲和层析从表鞭毛者中分离出分子量为65·8 kDa和59 kDa的HBP，并通过生物素结合的硫酸化糖胺聚糖（GAG）进行鉴定。表面等离子体共振生物传感器分析表明稳定的受体-配体结合基于关联和解离值。附睾前体与GAG的预孵育导致寄生虫与固定化肝素结合的抑制。进行竞争测定以评估HBP-GAG相互作用在表鞭毛象识别和粘附到罗得牛中肠上皮细胞中的作用。与HBP预孵育的上皮细胞对表鞭tig的粘附有3·8倍的抑制作用。用肝素，硫酸乙酰肝素和硫酸软骨素预处理表鞭毛鞭毛纲动物可显着抑制寄生虫粘附至中肠上皮细胞，这已通过扫描电子显微镜得到证实。我们提供的证据表明，肝素结合蛋白存在于克鲁斯锥T的表面上，并证明了它们在昆虫载体中肠上皮细胞表面的硫酸化GAGs识别中的关键作用。

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