...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Parasitology >Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany
【24h】

Molecular identification of Sarcocystis spp. helped to define the origin of green pythons (Morelia viridis) confiscated in Germany

机译：肉胞藻的分子鉴定。帮助定义了在德国没收的绿色蟒蛇（Morelia viridis）的起源

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sarcocystis spp. represent apicomplexan parasites. They usually have a heteroxenous life cycle. Around 200 species have been described, affecting a wide range of animals worldwide, including reptiles. In recent years, large numbers of reptiles have been imported into Europe as pets and, as a consequence, animal welfare and species protection issues emerged. A sample of pooled feces from four confiscated green pythons (Morelia viridis) containing Sarcocystis spp. sporocysts was investigated. These snakes were imported for the pet trade and declared as being captive-bred. Full length 18S rRNA genes were amplified, cloned into plasmids and sequenced. Two different Sarcocystis spp. sequences were identified and registered as Sarcocystis sp. from M. viridis in GenBank. Both showed a 95-97% sequence identity with the 18S rRNA gene of Sarcocystis singaporensis. Phylogenetic analysis positioned these sequences together with other Sarcocystis spp. from snakes and rodents as definitive and intermediate hosts (IH), respectively. Sequence data and also the results of clinical and parasitological examinations suggest that the snakes were definitive hosts for Sarcocystis spp. that circulate in wild IH. Thus, it seems unlikely that the infected snakes had been legally bred. Our research shows that information on the infection of snakes with Sarcocystis spp. may be used to assess compliance with regulations on the trade with wildlife species.
机译：肉囊藻属代表apicomplexan寄生虫。它们通常具有异质的生命周期。已经描述了大约200种，影响了全世界的各种动物，包括爬行动物。近年来，大量爬行动物被作为宠物进口到欧洲，结果出现了动物福利和物种保护问题。从四个被没收的含有Sarcocystis spp的绿色蟒蛇（莫雷利亚绿蝇）中收集的粪便样本。调查了孢子囊。这些蛇是为宠物贸易而进口的，并被宣布为人工饲养。扩增全长18S rRNA基因，克隆到质粒中并测序。两个不同的Sarcocystis spp。序列被鉴定并注册为Sarcocystis sp。来自GenBank中的M. viridis。两者均显示出与Sarcocystis singaporensis的18S rRNA基因具有95-97％的序列同一性。系统发育分析将这些序列与其他石藻属一起定位。来自蛇和啮齿动物，分别作为确定的宿主和中间宿主（IH）。序列数据以及临床和寄生虫学检查的结果表明，这些蛇是石藻属的确定宿主。在野生IH中传播。因此，受感染的蛇似乎不可能被合法繁殖。我们的研究表明，有关蛇肉被Sarcocystis spp感染的信息。可用于评估对野生动植物物种交易的遵守情况。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号