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首页> 外文期刊>Parasitology >Fasciola hepatica mucin-encoding gene: expression, variability and its potential relevance in host-parasite relationship.
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Fasciola hepatica mucin-encoding gene: expression, variability and its potential relevance in host-parasite relationship.

机译：Fasciola hepatica粘蛋白编码基因：在宿主-寄生虫关系中的表达，变异性及其潜在相关性。

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Fasciola hepatica is the causative agent of fasciolosis, a zoonosis with significant impact both in human and animal health. Understanding the basic processes of parasite biology, especially those related to interactions with its host, will contribute to control F. hepatica infections and hence liver pathology. Mucins have been described as important mediators for parasite establishment within its host, due to their key roles in immune evasion. In F. hepatica, mucin expression is upregulated in the mammalian invasive newly excysted juvenile (NEJ) stage in comparison with the adult stage. Here, we performed sequencing of mucin cDNAs prepared from NEJ RNA, resulting in six different cDNAs clusters. The differences are due to the presence of a tandem repeated sequence of 66 bp encoded by different exons. Two groups of apomucins one with three and the other with four repeats, with 459 and 393 bp respectively, were identified. These cDNAs have open reading frames encoding Ser-Thr enriched proteins with an N-terminal signal peptide, characteristic of apomucin backbone. We cloned a 4470 bp gene comprising eight exons and seven introns that encodes all the cDNA variants identified in NEJs. By real time polymerase chain reaction and high-resolution melting approaches of individual flukes we infer that fhemuc-1 is a single-copy gene, with at least two different alleles. Our data suggest that both gene polymorphism and alternative splicing might account for apomucin variability in the fhemuc-1 gene that is upregulated in NEJ invasive stage. The relevance of this variation in host-parasite interplay is discussed.
机译：肝片fasciola是fasciolosis的病原体，fasciolosis是对人和动物健康均具有重大影响的人畜共患病。了解寄生虫生物学的基本过程，特别是与宿主宿主相互作用有关的过程，将有助于控制肝炎性肝炎感染，进而控制肝脏病理。粘蛋白因其在免疫逃逸中的关键作用而被描述为在其宿主内建立寄生虫的重要介质。与成人期相比，在肝炎性肝炎中，粘蛋白表达在哺乳动物浸润性新近囊化幼虫（NEJ）期中被上调。在这里，我们对从NEJ RNA制备的粘蛋白cDNA进行了测序，得到了六个不同的cDNA簇。差异是由于存在由不同外显子编码的66 bp的串联重复序列。鉴定出了两组分别为459bp和393bp的阿朴粘蛋白，其中一组具有三个重复，而另一组具有四个重复。这些cDNA具有开放阅读框，其编码富含Apomucin主链特征的N端信号肽的富含Ser-Thr的蛋白质。我们克隆了一个包含8个外显子和7个内含子的4470 bp基因，该基因编码在NEJs中鉴定的所有cDNA变体。通过实时聚合酶链反应和单个吸虫的高分辨率解链方法，我们推断fhemuc-1是单拷贝基因，具有至少两个不同的等位基因。我们的数据表明，基因多态性和选择性剪接都可能解释了NEJ侵袭阶段上调的fhemuc-1基因中的载脂蛋白变异性。讨论了宿主-寄生虫相互作用中这种变化的相关性。

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