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首页> 外文期刊>Parasitology >Egg-specific expression of protein with DNA methyltransferase activity in the biocarcinogenic liver fluke Clonorchis sinensis
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Egg-specific expression of protein with DNA methyltransferase activity in the biocarcinogenic liver fluke Clonorchis sinensis

机译：生物致癌肝吸虫Cornorchis sinensis中具有DNA甲基转移酶活性的蛋的蛋白质特异性表达

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Despite recent reports regarding the biology of cytosine methylation in Schistosoma mansoni, the impact of the regulatory machinery remains unclear in diverse platyhelminthes. This ambiguity is reinforced by discoveries of DNA methyltransferase 2 (DNMT2)-only organisms and the substrate specificity of DNMT2 preferential to RNA molecules. Here, we characterized a novel DNA methyltransferase, named CsDNMT2, in a liver fluke Clonorchis sinensis. The protein exhibited structural properties conserved in other members of the DNMT2 family. The native and recombinant CsDNMT2 exhibited considerable enzymatic activity on DNA. The spatiotemporal expression of CsDNMT2 mirrored that of 5-methylcytosine (5 mC), both of which were elevated in the C. sinensis eggs. However, CsDNMT2 and 5 mC were marginally detected in other histological regions of C. sinensis adults including ovaries and seminal receptacle. The methylation site seemed not related to genomic loci occupied by progenies of an active long-terminal-repeat retrotransposon. Taken together, our data strongly suggest that C. sinensis has preserved the functional DNA methylation machinery and that DNMT2 acts as a genuine alternative to DNMT1/DNMT3 to methylate DNA in the DNMT2-only organism. The epigenetic regulation would target functional genes primarily involved in the formation and/or maturation of eggs, rather than retrotransposons.
机译：尽管最近有关于曼氏血吸虫中胞嘧啶甲基化生物学的报道，但在各种疟原虫中调节机制的影响仍不清楚。仅DNA甲基转移酶2（DNMT2）的生物的发现和DNMT2优先于RNA分子的底物特异性进一步增强了这种歧义。在这里，我们表征了一种新的DNA甲基转移酶，命名为CsDNMT2，在中华绒螯蟹中华吸虫中。该蛋白质表现出在DNMT2家族其他成员中保守的结构特性。天然的和重组的CsDNMT2对DNA表现出相当大的酶活性。 CsDNMT2的时空表达与5-甲基胞嘧啶（5 mC）的时空表达相似，两者在中华绒螯蟹卵中均升高。然而，CsDNMT2和5 mC在中华绒螯蟹成虫的其他组织学区域（包括卵巢和精囊）中被少量检测到。甲基化位点似乎与活跃的长末端重复逆转录转座子的后代所占据的基因组位点无关。两者合计，我们的数据强烈表明中华梭菌保留了功能性的DNA甲基化机制，并且DNMT2可以作为DNMT1 / DNMT3的真正替代品，在仅DNMT2的生物中甲基化DNA。表观遗传调控将靶向主要参与卵的形成和/或成熟的功能基因，而不是逆转座子。

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