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首页> 外文期刊>Parasitology >Chronic infection of Toxoplasma gondii downregulates miR-132 expression in multiple brain regions in a sex-dependent manner
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Chronic infection of Toxoplasma gondii downregulates miR-132 expression in multiple brain regions in a sex-dependent manner

机译：弓形虫的慢性感染以性别依赖性方式下调多个大脑区域的miR-132表达

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摘要

MicroRNA-132 (miR-132) has been demonstrated to affect multiple neuronal functions and its dysregulation is linked to several neurological disorders. We previously showed that acute Toxoplasma gondii infection induces miR-132 expression both in vitro and in vivo. To investigate the impact of chronic infection on miR-132, we infected mice with T. gondii PRU strain and performed assessment 5 months later in six brain regions (cortex, hypothalamus, striatum, cerebellum, olfactory bulb and hippocampus) by qPCR. We found that while acute infection of T. gondii increases the expression of miR-132, chronic infection has the opposite effect. The effect varied amongst different regions of the brain and presented in a sex-dependent manner, with females exhibiting more susceptibility than males. MiR-132 and brain-derived neurotrophic factor (BDNF, an inducer of miR-132) were not co-varies in the brain areas of infected mice. T. gondii DNA/RNA was found in all tested brain regions and a selective tropism towards the hippocampus, based on bradyzoite density, was observed in both males and females. However, the expressions of miR-132 or BDNF were poorly reflected by the density of T. gondii in brain areas. Our findings highlight the importance of investigating the miR-132-mediated neuronal function in mice infected with T. gondii.
机译：MicroRNA-132（miR-132）已被证明会影响多种神经元功能，其失调与多种神经系统疾病有关。我们先前显示，急性弓形虫感染可在体外和体内诱导miR-132表达。为了研究慢性感染对miR-132的影响，我们用弓形虫PRU株感染了小鼠，并于5个月后通过qPCR在六个大脑区域（皮质，下丘脑，纹状体，小脑，嗅球和海马体）进行了评估。我们发现，虽然弓形虫的急性感染增加了miR-132的表达，但慢性感染却具有相反的作用。这种作用在大脑的不同区域之间是不同的，并且表现出性别依赖性，女性比男性表现出更高的敏感性。在受感染小鼠的大脑区域，MiR-132和脑源性神经营养因子（BDNF，miR-132的诱导剂）不是协变量。在所有测试的大脑区域都发现了弓形虫DNA / RNA，并且在雄性和雌性中均观察到了基于缓殖子密度的对海马的选择性向性。然而，miR-132或BDNF的表达不能很好地反映出弓形虫在大脑区域的密度。我们的发现突出了研究感染弓形虫的小鼠中miR-132介导的神经元功能的重要性。

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