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首页> 外文期刊>Parasitology Research >Microsatellite marker analysis shows differentiation among Trypanosoma cruzi populations of peripheral blood and dejections of Triatoma infestans fed on the same chronic chagasic patients : microsatellite marker analysis and T. cruzi.
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Microsatellite marker analysis shows differentiation among Trypanosoma cruzi populations of peripheral blood and dejections of Triatoma infestans fed on the same chronic chagasic patients : microsatellite marker analysis and T. cruzi.

机译：微卫星标记分析显示，在同一慢性恰加斯病患者中，克氏锥虫外周血种群之间存在分化，并感染了Triatoma infestans的排斥反应：微卫星标记分析和克氏锥虫。

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摘要

To investigate whether Trypanosoma cruzi populations found in chagasic cardiopathic and non-cardiopathic patients are genetically differentiated, three molecular microsatellite markers were analysed. This analysis was also applied to compare T. cruzi samples from peripheral blood or dejections of Triatoma infestans fed on the blood of the same patients. In order to obtain the first objective, analyses of predominant T. cruzi genotypes were conducted using three approaches: a locus-by-locus analysis; a Fisher method across three loci; and analysis of molecular variance by Genepop and Arlequin programs. Only with one locus and on the blood samples was a significant differentiation detected among non-cardiopathic and cardiopathic groups, which was not confirmed by the other two methods. On the contrary, with the three approaches, it was found that T. cruzi clones present in the blood of patients are genetically differentiated from those detected in dejections of T. infestans fed on the same patients. Our results showed that the most frequent lineage both in blood as well as in triatomine dejection samples was TcI. No significant difference in T. cruzi lineage distribution was observed among chagasic cardiopathic and non-cardiopathic patients. The majority of the samples (50-60%) had only one T. cruzi clone (uniclonal) either in blood or dejection samples.
机译：为了研究是否在遗传性遗传上区分了在chagasic心脏病和非心脏病患者中发现的克鲁氏锥虫种群，对三种分子微卫星标记进行了分析。这项分析还用于比较来自同一患者血液的外周血或感染的Triatoma infestans的粪便中的克鲁氏锥虫样品。为了获得第一个目标，使用三种方法对主要的克鲁维氏锥虫基因型进行了分析：逐位点分析;跨越三个位点的Fisher方法;通过Genepop和Arlequin程序进行分子变异分析。仅在一个部位和血液样本上，在非心病和心病组之间检测到显着差异，而其他两种方法未确认。相反，通过这三种方法，发现存在于患者血液中的克氏锥虫克隆与以相同患者为基础的感染无锡锥虫的沮丧中检测到的具有遗传差异。我们的研究结果显示，血液以及三氢精氨酸抑制样品中最常见的血统是TcI。在chagasic心脏病患者和非心病患者中，T。cruzi谱系分布没有显着差异。大部分样本（50％至60％）在血液或粪便样本中只有一个克鲁氏梭菌克隆（单克隆）。

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