IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ENTOMOPATHOGENIC NEMATODES (STEINERNEMATIDAEAND HETERORHABDITIDAE) FROM PAKISTAN AS INFERRED FROM RIBOSOMAL DNA SEQUENCES OF THE ITS1-5.8S-ITS2 REGION

F. SHAHINA; X. YAN; R. HAN; G. MEHREEN

首页> 外文期刊>Pakistan Journal of Nematology >IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ENTOMOPATHOGENIC NEMATODES (STEINERNEMATIDAEAND HETERORHABDITIDAE) FROM PAKISTAN AS INFERRED FROM RIBOSOMAL DNA SEQUENCES OF THE ITS1-5.8S-ITS2 REGION

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IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ENTOMOPATHOGENIC NEMATODES (STEINERNEMATIDAEAND HETERORHABDITIDAE) FROM PAKISTAN AS INFERRED FROM RIBOSOMAL DNA SEQUENCES OF THE ITS1-5.8S-ITS2 REGION

机译：从ITS1-5.8S-ITS2区核糖体DNA序列推论巴基斯坦的致病性线虫（铁线虫和异戊二烯）的鉴定和系统发育分析

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The ITS1-5.8S-ITS2 regions of rDNA of 9 strains from Pakistan were PCR amplified and cloned. Sequences of these fragments were analyzed and phylogenetic trees were constructed with Caenorhabditis elegans as out group. Phylogenetic relationships amongthese strains were estimated. One of these strains MM7 belongs to Heterorhabditis and phylogenetic analysis showed the maximum similarity with H. indica. The other 8 strains were Steinernema. Phylogenetic analysis showed that strains M24, M33, G6, G98 and K8 belong to S. siamkayai, and strain 105, 109 were S. pakistanense, while strain T51 was close to S. carpocapsae.

机译：PCR扩增并克隆了来自巴基斯坦的9个菌株的rDNA的ITS1-5.8S-ITS2区域。分析这些片段的序列，并以秀丽隐杆线虫为外群构建系统发育树。估计了这些菌株之间的亲缘关系。这些菌株MM7中的一种属于异种哈氏杆菌，系统发育分析显示它与in稻的最大相似性。其他8株是Steinernema。系统进化分析表明，菌株M24，M33，G6，G98和K8属于S. siamkayai，菌株105、109为巴基斯坦链霉菌，而菌株T51接近于拟糖链霉菌。

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《Pakistan Journal of Nematology》 |2010年第1期|共19页
作者
F. SHAHINA; X. YAN; R. HAN; G. MEHREEN;
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中图分类昆虫学;
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1. IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ENTOMOPATHOGENIC NEMATODES (STEINERNEMATIDAEAND HETERORHABDITIDAE) FROM PAKISTAN AS INFERRED FROM RIBOSOMAL DNA SEQUENCES OF THE ITS1-5.8S-ITS2 REGION [J] . F. SHAHINA, X. YAN, R. HAN, Pakistan Journal of Nematology . 2010,第1期

机译：从ITS1-5.8S-ITS2区核糖体DNA序列推论巴基斯坦的致病性线虫（铁线虫和异戊二烯）的鉴定和系统发育分析
2. First record of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Nigerian soil and their morphometrical and ribosomal DNA sequence analysis. [J] . Akyazi F., Ansari M. A., Ahmed B. I., Nematologia Mediterranea . 2012,第2期

机译：尼日利亚土壤中的昆虫病原线虫（Steinernematidae和Heterorhabditidae）的首次记录及其形态和核糖体DNA序列分析。
3. Phylogenetic Relationships within the Cyst-Forming Nematodes (Nematoda, Heteroderidae) Based on Analysis of Sequences from the ITS Regions of Ribosomal DNA [J] . Sergei A. Subbotin, Andy Vierstraete, Paul De Ley, Molecular phylogenetics and evolution . 2001,第1期

机译：基于核糖体DNA ITS区序列的囊形成线虫（线虫，异翅目科）内的亲缘关系
4. BIOLUMINESCENCE IN CLICK BEETLES (FAMILY ELATERIDAE): MOLECULAR PHYLOGENETIC ANALYSIS, INFERRED FROM 28S RIBOSOMAL DNA [C] . REIKO SAGEGAMI-OBA, YUICHI OBA, HITOO OHIRA International Symposium on Bioluminescence and Chemiluminescence . 2007

机译：单击甲虫（家庭Elateridae）的生物发光：分子系统发育分析，从28S核糖体DNA推断出来
5. Phylogenetic relationships of the North American phoxinins (Actinopterygii: Cyprinidae) inferred from mitochondrial ribosomal DNA sequences [D] . Simons, Andrew Michael 1997

机译：从线粒体核糖体DNA序列推论的北美phoxinins（放线菌纲：Cyprinidae）的亲缘关系。
6. Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii [O] . Dimitri V Ghikas, Vassili N Kouvelis, Milton A Typas 2010

机译：线粒体基因间区域分析和昆虫致病性球孢白僵菌和B. brongniartii的ITS1-5.8S-ITS2推断的系统发生和生物地理意义。
7. Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii [O] . Typas Milton A, Kouvelis Vassili N, Ghikas Dimitri V 2010

机译：线粒体基因间区域分析和昆虫病原真菌白僵菌和白僵菌的ITS1-5.8S-ITS2推断出系统发生学和生物地理学意义。 brongniartii

IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ENTOMOPATHOGENIC NEMATODES (STEINERNEMATIDAEAND HETERORHABDITIDAE) FROM PAKISTAN AS INFERRED FROM RIBOSOMAL DNA SEQUENCES OF THE ITS1-5.8S-ITS2 REGION

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