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首页> 外文期刊>Pakistan Journal of Nematology >New host record of root-knot nematode (Meloidogyne graminicola) in Pakistan
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New host record of root-knot nematode (Meloidogyne graminicola) in Pakistan

机译：巴基斯坦的根结线虫（Meloidogyne graminicola）的新寄主记录

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摘要

A survey of rice growing areas in two districts of Punjab, Faisalabad and Chiniot was conducted during September 2014-15 for root-knot nematode, Meloidogyne graminicola Golden & Brichfield, 1965. During survey, typical symptoms of hook like galls on roots were observed not only in rice plants but also in weeds. Root samples were examined in plant nematology laboratory in Department of Plant Pathology, University of Agriculture, Faisalabad. Roots of different weeds; Cyperus rotundus, Dactyloctenium aegyptium, Echinochloa crusgalli, Eclipta alba, Paspalum distichum from rice field and Avena fatua, Phalaris minor and Rumex dentatus from wheat field were collected during summer and winter 2014-15, respectively. Heavy galling on roots indicated the root-knot nematodes infestation. Roots of each plant were separated according to gall structure and mature nematode females were isolated under stereomicroscope through dissection. From each plant, five samples of roots and ten nematode females were examined.Impression of perineal patterns of each female were prepared and identified. Mixed population of nematode was found in A. fatua, R. dentatus and P. minor. It was observed that more than one type of root galls in a single plant root system were present and differentiated on the basis of gall shape and egg-mass positions. All weed plants showed clear symptoms of M.graminicola on their root that was confirmed through perennial pattern (Eisenback et al., 1981). The other root-knot species confirmed was M. arenaria (Neal, 1889) Chitwood, 1949. These are new host records of root-knot nematode from Punjab, Pakistan.
机译：2014年9月至15日，对旁遮普邦，费萨拉巴德和奇尼奥特两个地区的水稻种植区进行了调查，调查了根结线虫Meloidogyne graminicola Golden＆Brichfield，1965年。在调查中，未观察到根系上有钩子状的典型症状不仅在水稻植物中，而且在杂草中。根样品在费萨拉巴德农业大学植物病理学系的植物线虫学实验室检查。不同杂草的根;在2014-15年夏季和冬季，分别从稻田和麦田的Avena fatua，圆麦，香根草，cru草，Eclipta alba，Paspalum distichum，麦草的Phalaris和Rumex dentatus中收集了香附子。根部有大量刺痕，表明根结线虫为患。根据胆汁结构分离每株植物的根，并在立体显微镜下通过解剖分离出成熟的线虫雌性。从每棵植物中检查了5个根和10个线虫雌性样品，并制备并鉴定了每个雌性会阴模式的印象。在A. fatua，R。dentatus和P. minor中发现了线虫的混合种群。据观察，在单个植物的根系中存在不止一种类型的根gall，并根据and的形状和卵的质量位置进行区分。所有杂草植物在其根部均显示出明显的M.graminicola症状，并通过多年生模式得以证实（Eisenback等，1981）。确认的其他根结线虫种是沙雷氏菌（Neal，1889年），奇特伍德，1949年。这些是巴基斯坦旁遮普邦的根结线虫的新寄主记录。

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