...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomaterials >Hydrogels based on poly(ethylene oxide) and poly(tetramethylene oxide) or poly(dimethyl siloxane): synthesis, characterization, in vitro protein adsorption and platelet adhesion.
【24h】

Hydrogels based on poly(ethylene oxide) and poly(tetramethylene oxide) or poly(dimethyl siloxane): synthesis, characterization, in vitro protein adsorption and platelet adhesion.

机译：基于聚环氧乙烷和聚四氢呋喃或聚二甲基硅氧烷的水凝胶：合成，表征，体外蛋白质吸附和血小板粘附。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In vitro protein adsorption, platelet adhesion and activation on new hydrogel surfaces, composed of poly(ethylene oxide) (PEO) and poly(tetramethylene oxide) (PTMO) or poly(dimethyl siloxane) (PDMS), were investigated. By varying PEO length (MW = 2000 or 3400), hydrophobic components (PTMO or PDMS) or polymer topology (block or graft copolymers), various physical hydrogels were produced. Their structures were verified by 1H NMR and ATR-IR and the molecular weights were determined by gel permeation chromatography. The hydrogels were soluble in a variety of organic solvents, while absorbed a significant amount of water with preserved three-dimensional structure by physical crosslinking. The dynamic contact angle measurement revealed that the surface hydrophilicity increased by incorporating longer PEO, PEO grafting, and adopting PDMS as a hydrophobic segment instead of PTMO. It was observed from in vitro protein adsorption study that the hydrogels exhibited significantly lower adsorption of human serum albumin (HSA), human fibrinogen (HFg), and IgG, when compared with Pellethane, a commercial polyurethane taken as a control. The hydrogels were attractive for HSA but not sensitive to HFg and IgG. And more than 65% of the proteins detected on the surfaces of the hydrogels were reversibly detached by being treated with an SDS solution. It was evident that the hydrogels synthesized in this study were much more resistant to platelet adhesion than the control, which might depend on the composition of proteins adsorbed on the surfaces and their degree of denaturation. Among the hydrogels tested, PEO3,4kPDMS exhibited albumin-rich and platelet-resistant surfaces, implying a potential candidate for biomaterial.
机译：研究了由聚（环氧乙烷）（PEO）和聚（环氧丙烷）（PTMO）或聚（二甲基硅氧烷）（PDMS）组成的新型水凝胶表面上的蛋白质体外吸附，血小板粘附和活化。通过改变PEO长度（MW = 2000或3400），疏水性组分（PTMO或PDMS）或聚合物拓扑结构（嵌段或接枝共聚物），可以生产出各种物理水凝胶。它们的结构通过1 H NMR和ATR-IR验证，分子量通过凝胶渗透色谱法测定。水凝胶可溶于多种有机溶剂，同时通过物理交联吸收了大量水，并保留了三维结构。动态接触角测量表明，通过结合更长的PEO，PEO接枝并采用PDMS作为疏水链段代替PTMO，可以提高表面亲水性。从体外蛋白质吸附研究中观察到，与作为对照的商业聚氨酯Pellethane相比，水凝胶对人血清白蛋白（HSA），人纤维蛋白原（HFg）和IgG的吸附明显降低。水凝胶对HSA具有吸引力，但对HFg和IgG不敏感。通过用SDS溶液处理，可逆分离地分离出超过65％的水凝胶表面检测到的蛋白质。显然，在这项研究中合成的水凝胶比对照对血小板粘附的抵抗力强得多，这可能取决于吸附在表面的蛋白质的组成及其变性程度。在测试的水凝胶中，PEO3,4kPDMS表现出富含白蛋白和抗血小板的表面，这暗示了生物材料的潜在候选者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号