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首页> 外文期刊>Pedobiologia >Secondary structure and sequence variation of the 28S rRNA gene in the Neanuridae, and its utility as a phylogenetic marker
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Secondary structure and sequence variation of the 28S rRNA gene in the Neanuridae, and its utility as a phylogenetic marker

机译：线虫中28S rRNA基因的二级结构和序列变异及其作为系统发生标记的用途

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摘要

A fragment of the 28S rRNA encompassing the divergent domains D1-D5 was sequenced in 14 species of Collembola Neanuridae, 12 of which belong to the subfamily Neanurinae. A slight length variation across taxa was found in the D2, D3 and D5 divergent domains, the core regions being very conserved in length and sequence. Nucleotide composition was slightly biased towards As and Ts (55.6%), with the bias being more evident in the nucleotides not involved in pairing with complementary sequences. A+T bias was higher in the divergent domains than in the core regions. The number of variable sites was high in the divergent domains, and positively correlated to their length, with the D2 domain being the longest and the most variable one. Phylogenetic analysis strongly suggested monophyly of the Neanurinae and less strongly of the Neanurini. It also suggested polyphyly of the genera Neanura and Delltoura. Some relationships are in contrast with morphological evidences, but are in agreement with molecular data from the mitochondrial COII gene.
机译：在14个种类的Collembola Neanuridae中，对28S rRNA的一个片段进行了测序，该片段包含D1-D5的不同结构域，其中12个属于Neanurinae亚科。在D2，D3和D5差异域中发现整个类群的长度略有变化，核心区域的长度和序列非常保守。核苷酸组成稍微偏向As和Ts（55.6％），这种偏斜在不参与与互补序列配对的核苷酸中更为明显。在差异域中，A + T偏向高于核心区域。在不同的域中可变位点的数量高，并且与它们的长度成正相关，其中D2域是最长和可变性最大的一个。系统发育分析强烈暗示了神经鞭毛虫的单亲性，而对神经鞭毛虫的抵抗力较弱。它也暗示了Neanura和Delltoura属的多重特征。某些关系与形态学证据相反，但与线粒体COII基因的分子数据一致。

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