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首页> 外文期刊>Biomaterials >A bFGF-releasing silk/PLGA-based biohybrid scaffold for ligament/tendon tissue engineering using mesenchymal progenitor cells.
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A bFGF-releasing silk/PLGA-based biohybrid scaffold for ligament/tendon tissue engineering using mesenchymal progenitor cells.

机译：一种bFGF释放丝/基于PLGA的生物混合支架，用于使用间充质祖细胞进行韧带/肌腱组织工程。

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摘要

An ideal scaffold that provides a combination of suitable mechanical properties along with biological signals is required for successful ligament/tendon regeneration in mesenchymal stem cell-based tissue engineering strategies. Among the various fibre-based scaffolds that have been used, hybrid fibrous scaffolds comprising both microfibres and nanofibres have been recently shown to be particularly promising. This study developed a biohybrid fibrous scaffold system by coating bioactive bFGF-releasing ultrafine PLGA fibres over mechanically robust slowly-degrading degummed knitted microfibrous silk scaffolds. On the ECM-like biomimetic architecture of ultrafine fibres, sustained release of bFGF mimicked the ECM in function, initially stimulating mesenchymal progenitor cell (MPC) proliferation, and subsequently, their tenogeneic differentiation. The biohybrid scaffold system not only facilitated MPC attachment and promoted cell proliferation, with cells growing both on ultrafine PLGA fibres and silk microfibres, but also stimulated tenogeneic differentiation of seeded MPCs. Upregulated gene expression of ligament/tendon-specific ECM proteins and increased collagen production likely contributed to enhancing mechanical properties of the constructs, generating a ligament/tendon analogue that has the potential to be used to repair injured ligaments/tendons.
机译：在基于间充质干细胞的组织工程策略中，成功的韧带/肌腱再生需要理想的支架，该支架必须提供适当的机械性能以及生物学信号的组合。在已使用的各种基于纤维的支架中，包括微纤维和纳米纤维的混合纤维支架最近已被证明是特别有前途的。这项研究通过将释放生物活性bFGF的超细PLGA纤维涂覆在机械坚固，缓慢降解的脱胶针织微纤维丝支架上，从而开发了生物杂化纤维支架系统。在超细纤维的类似ECM的仿生结构上，bFGF的持续释放模仿了ECM的功能，最初刺激了间充质祖细胞（MPC）的增殖，随后刺激了它们的遗传分化。生物杂交支架系统不仅促进了MPC的附着并促进了细胞的增殖，细胞在超细PLGA纤维和丝微纤维上生长，而且还刺激了种子MPC的遗传分化。韧带/肌腱特异性ECM蛋白的基因表达上调和胶原蛋白生成的增加可能有助于增强构建体的机械性能，从而产生了一种韧带/肌腱类似物，可用于修复受伤的韧带/肌腱。

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