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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Depletion of tumor-infiltrating macrophages is associated with amphoterin expression in colon cancer.
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Depletion of tumor-infiltrating macrophages is associated with amphoterin expression in colon cancer.

机译：肿瘤浸润性巨噬细胞的耗竭与结肠癌中的两性蛋白表达有关。

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Macrophage infiltration into colon cancer and amphoterin expression in cancer cells was examined in 42 human colon cancers invading the subserosa. The mean number of infiltrating macrophages was significantly higher in Dukes' B cases than in Dukes' C cases (p = 0.0065). Tumors with few infiltrating macrophages (macrophage depletion) were significantly more frequent in Dukes' C cases than in Dukes' B cases (p = 0.0014). No Dukes' C cases with relevant macrophage infiltration showed macrophage-cancer cell contact, whereas 5 Dukes' B cases showed such contact (p < 0.0001). In human colon cancer cells implanted in the cecum of nude mice, KM12SM (highly metastatic) tumors yielded less macrophage infiltration and more liver metastases than KM12C (low risk of metastasis) tumors (14 +/- 3 vs. 78 +/- 32 and 24 +/- 6 vs. 5 +/- 3 per liver, respectively). Amphoterin expression was detected at high frequency in both Dukes' B and C cases (p = 0.0684). In macrophage-depleted cases, amphoterin expression was significantly higher than that in non-depleted cases (p = 0.0015). To confirm biological effects of amphoterin on macrophages, an infiltration assay using the cell-layered Boyden chamber was done. Infiltration of PMA-treated U937 monocytes through the KM12SM cell layer was increased by pretreatment of KM12SM cells with amphoterin antisense S-oligodeoxynucleotide exposure. Moreover, extracted amphoterin inhibited PMA-U937 monocyte infiltration in a dose-dependent manner. Thus, amphoterin may play an important role in the inhibition of macrophage infiltration into colon cancer.
机译：在侵袭浆膜下层的42例人类结肠癌中检查了巨噬细胞浸润到结肠癌中和两性霉素在癌细胞中的表达。 Dukes'B病例的浸润巨噬细胞平均数显着高于Dukes'C病例（p = 0.0065）。 Dukes C例中浸润性巨噬细胞很少（巨噬细胞耗竭）的肿瘤比Dukes'B例中更为常见（p = 0.0014）。没有相关巨噬细胞浸润的Dukes'C病例显示巨噬细胞-癌细胞接触，而5个Dukes'B病例显示有这种接触（p <0.0001）。在植入裸鼠盲肠的人结肠癌细胞中，KM12SM（高度转移性）肿瘤比KM12C（低转移风险）肿瘤产生更少的巨噬细胞浸润和更多的肝转移（14 +/- 3 vs. 78 +/- 32 and每个肝脏分别为24 +/- 6和5 +/- 3）。在Dukes的B和C病例中均以高频率检测到两性霉素表达（p = 0.0684）。在巨噬细胞缺乏的情况下，两性霉素的表达显着高于未耗尽的病例（p = 0.0015）。为了证实两性霉素对巨噬细胞的生物学作用，使用细胞层的博登室进行了浸润测定。通过用两性蛋白反义S-寡脱氧核苷酸暴露预处理KM12SM细胞，可以增加PMA处理的U937单核细胞通过KM12SM细胞层的渗透。此外，提取的两性霉素以剂量依赖的方式抑制PMA-U937单核细胞浸润。因此，两性霉素可能在抑制巨噬细胞浸入结肠癌中起重要作用。

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