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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Molecular analysis of nipple fluid for breast cancer screening.
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Molecular analysis of nipple fluid for breast cancer screening.

机译：用于乳癌筛查的乳头液分子分析。

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Lack of sensitivity and specificity of image-based breast cancer screening has urged the exploration of alternate screening modalities. Nipple fluid, which contains breast epithelial cells, is produced in small amounts in the breast ducts of nonlactating women and can be collected by noninvasive vacuum aspiration. After administration of nasal oxytocin, nipple aspiration yields sufficient material for molecular analysis in the large majority of women. Whereas nipple fluid cytology appears to have only a moderate correlation with breast cancer development, methylation holds promise as a more appropriate biomarker, since methylation aberrations occur as an early and frequent event during carcinogenesis. Using quantitative multiplex methylation-specific PCR, methylation can be detected in minute amounts of DNA extracted from nipple aspirates, precluding the need for more invasive intraductal approaches such as ductal lavage and random periareolar fine needle aspiration. The application of genomic and proteomic diagnostics to nipple aspirates therefore provides unprecedented opportunities for early breast cancer diagnosis amendable to population-based screening.
机译：基于图像的乳腺癌筛查的敏感性和特异性不足，促使人们探索其他筛查方式。非泌乳妇女的乳腺导管中会少量产生含有乳腺上皮细胞的乳头液，可通过无创真空抽吸术收集。鼻内催产素给药后，乳头抽吸可为绝大多数女性提供足够的分子分析材料。乳头液细胞学似乎与乳腺癌的发展只有中等程度的相关性，而甲基化有望成为更合适的生物标志物，因为甲基化畸变是在癌变过程中的早期和频繁事件。使用定量多重甲基化特异性PCR，可以从乳头抽吸物中提取微量DNA来检测甲基化，从而无需使用更具侵入性的导管内方法，例如导管灌洗和乳晕周围细针抽吸。因此，将基因组学和蛋白质组学诊断技术应用于乳头抽吸物，为早期乳腺癌诊断提供了前所未有的机会，并且可以进行基于人群的筛查。

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