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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >A novel CD11c monoclonal antibody effective in formalin-fixed tissue for the diagnosis of hairy cell leukemia.
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A novel CD11c monoclonal antibody effective in formalin-fixed tissue for the diagnosis of hairy cell leukemia.

机译：在福尔马林固定的组织中有效的新型CD11c单克隆抗体，可用于诊断毛细胞白血病。

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OBJECTIVE: The diagnosis of hairy cell leukemia (HCL) and its distinction from other B-cell lymphomas can be difficult in formalin-fixed tissue. This is because the histology is not always classical, and despite having a characteristic phenotype, there are few relevant monoclonal antibodies with sufficient sensitivity and specificity that can be applied to fixed material. In this study, we assessed the utility of a newly developed antibody against a formalin-resistant CD11c epitope (5D11) for the diagnosis and monitoring of HCL in formalin-fixed tissue. METHODS: We performed immunohistochemical staining for CD11c expression in formalin-fixed and also decalcified tissue of 196 small B-cell lymphomas, including 104 cases of HCL showing extensive to minimal infiltrates. RESULTS: The CD11c antibody was both sensitive and specific for HCL, even in cases with minimal infiltration. CONCLUSION: We recommend that this CD11c antibody be added to a panel of antibodies for immunostaining of formalin-fixed material, for differentiation of HCL from other small B-cell lymphomas, and for detection of residual disease following therapy.
机译：目的：在福尔马林固定的组织中，很难诊断毛细胞白血病（HCL）及其与其他B细胞淋巴瘤的区别。这是因为组织学并不总是经典的，并且尽管具有特征性的表型，但几乎没有可用于固定材料的具有足够敏感性和特异性的相关单克隆抗体。在这项研究中，我们评估了针对福尔马林抗性CD11c表位（5D11）的新开发抗体在福尔马林固定组织中HCL的诊断和监测中的效用。方法：我们对196例小B细胞淋巴瘤的福尔马林固定和脱钙组织中的CD11c表达进行了免疫组织化学染色，其中104例HCL浸润范围从大到小。结果：即使在浸润最少的情况下，CD11c抗体也对HCL敏感且具有特异性。结论：我们建议将该CD11c抗体添加到一组用于福尔马林固定材料的免疫染色，从其他小B细胞淋巴瘤中区分HCL以及检测治疗后残留疾病的抗体中。

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