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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Oligophrenin-1 is associated with cell adhesion and migration in prostate cancer
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Oligophrenin-1 is associated with cell adhesion and migration in prostate cancer

机译：Oligophrenin-1与前列腺癌的细胞黏附和迁移有关

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摘要

Objective: We performed Escherichia coli ampicillin secretion trap (CAST) analysis in prostate cancer (PCa) to identify novel biomarkers. We show here that OPHN1 , which encodes oligophrenin-1 protein, is upregulated in PCa. OPHN1 was first determined to be one of the genes associated with Xlinked mental retardation; however, neither the gene's function nor the link between its expression and survival of patients has been investigated.Methods: We investigate the expression of oligophrenin-1 in 141 PCa tissue samples by immunohistochemistry and perform functional analysis using RNA interference.Results: Immunohistochemical analysis of oligophrenin-1 demonstrated that 60 (43%) PCa cases were positive for oligophrenin-1. Positive oligophrenin-1 expression was significantly correlated with a high Gleason score (p = 0.0198). Furthermore, patients with oligophrenin- 1-positive PCa had a worse biochemical recurrence-free survival rate than patients with oligophrenin-1-negative PCa (p = 0.0079). Cell adhesion to fibronectin was significantly reduced in OPHN1 small interfering (si)RNA-transfected LNCaP and PC3 cells in comparison to negative-control siRNA-transfected cells. Knockdown of OPHN1 reduced the expression of ITGA5 and stress fiber formation in LNCaP and PC3 cells.Conclusion: These results suggest that oligophrenin-1 is involved in tumor progression in PCa.
机译：目的：我们在前列腺癌（PCa）中进行了大肠杆菌氨苄青霉素分泌陷阱（CAST）分析，以鉴定新的生物标志物。我们在这里显示，OPHN1，其编码寡聚肾素-1蛋白，在PCa中被上调。首先确定OPHN1是与X连锁智力低下有关的基因之一。方法：采用免疫组化方法检测141例PCa组织中oligophrenin-1的表达，并利用RNA干扰技术进行功能分析。结果：未检测到该基因的功能及其与患者生存之间的联系。 oligophrenin-1证实60例（43％）PCa病例为oligophrenin-1阳性。 oligophrenin-1阳性表达与高Gleason评分显着相关（p = 0.0198）。此外，低肾素-1阳性PCa患者比低肾素-1阴性PCa患者的无生化复发率差（p = 0.0079）。与阴性对照siRNA转染的细胞相比，在OPHN1小干扰（si）RNA转染的LNCaP和PC3细胞中，细胞对纤连蛋白的粘附显着降低。敲低OPHN1可以降低LNCaP和PC3细胞中ITGA5的表达并减少应力纤维的形成。结论：这些结果表明寡聚肾上腺素1参与PCa的肿瘤进展。

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