首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells.
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Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells.

机译:鼠腹腔巨噬细胞源性树突状细胞的抗原呈递。

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摘要

Murine peritoneal cavity macrophage derived dendritic cells (PEC-DC) generated using early growth factors, interleukin 4 and granulocyte-macrophage colony-stimulating factor followed by maturation in interferon-gamma plus either, Toxoplasma lysate antigen (TLA) or lipopolysaccharide, bind TLA by a nonspecific mechanism and continue to express major histocompatibility complex class II antigens after 24 h of culture in vitro. Moreover, the proliferation of CD3+ spleen T cells from mice immunized with Toxoplasma gondii homogenate, induced by PEC-DC-mediated antigen presentation was statistically significant and of consistent amplitude. This accessory function of PEC-DC is antigen specific.
机译:鼠腹腔巨噬细胞衍生的树突状细胞(PEC-DC)使用早期生长因子,白介素4和粒细胞巨噬细胞集落刺激因子生成,然后在干扰素-γ加上弓形虫溶胞产物抗原(TLA)或脂多糖中成熟,通过TLA结合TLA这是一种非特异性机制,在体外培养24小时后仍会继续表达主要的组织相容性复合物II类主要抗原。此外,PEC-DC介导的抗原呈递诱导的弓形虫匀浆免疫小鼠的CD3 +脾T细胞增殖具有统计学意义,且幅度一致。 PEC-DC的这种辅助功能是抗原特异性的。

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