• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Involvement of cAMP response element-binding protein activation in salivary secretion.
【24h】

Involvement of cAMP response element-binding protein activation in salivary secretion.

机译：cAMP反应元件结合蛋白活化参与唾液分泌。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

OBJECTIVE: Saliva secretion is mediated by cAMP and the calcium signaling pathway in salivary acinar cells. The PKA signaling pathway plays an important role in protein secretion through the activation of cAMP, in fluid secretion through the elevation of intracellular calcium and in the activation of cAMP response element-binding protein (CREB), which is involved in these signaling cascades. In this study, we investigated whether the activation of CREB plays a part in the salivary secretion in mice. METHODS: We examined CREB activation by assessing phosphorylation at the serine-133 position using Western blotting. RESULTS: Carbachol (a muscarinic acetylcholine agonist) and isoproterenol (a beta-adrenergic agonist) markedly activated CREB in parotid acinar cells. Carbachol and isoproterenol-induced CREB phosphorylation was blocked by atropine (a muscarinic acetylcholine antagonist) and propranolol (a beta-adrenergic antagonist), respectively. The PKA inhibitor H89 inhibited CREB activation, but the PLC inhibitor U73122 did not. Moreover, carbachol- and isoproterenol-stimulated amylase secretion from parotid acinar cells was inhibited by H89 and adenoviral dominant-negative CREB. CONCLUSION: These results indicate that the muscarinic and beta-adrenergic activation of CREB was mediated through the PKA pathway and that CREB is involved in protein secretion from parotid acinar cells.
机译：目的：唾液腺分泌是由唾液腺腺癌细胞中的cAMP和钙信号通路介导的。 PKA信号通路在激活cAMP的蛋白质分泌，通过细胞内钙升高的液体分泌以及cAMP反应元件结合蛋白（CREB）的激活中起着重要作用，这些信号级联反应中都涉及到。在这项研究中，我们调查了CREB的激活是否在小鼠唾液分泌中起作用。方法：我们通过使用蛋白质印迹评估丝氨酸133位的磷酸化来检查CREB的活化。结果：卡巴胆碱（毒蕈碱型乙酰胆碱激动剂）和异丙肾上腺素（β-肾上腺素能激动剂）在腮腺腺泡细胞中显着激活了CREB。卡巴胆碱和异丙肾上腺素诱导的CREB磷酸化分别被阿托品（毒蕈碱乙酰胆碱拮抗剂）和普萘洛尔（β-肾上腺素拮抗剂）阻断。 PKA抑制剂H89抑制CREB活化，而PLC抑制剂U73122则不。此外，H89和腺病毒显性阴性CREB抑制了腮腺腺泡细胞中卡巴胆碱和异丙肾上腺素刺激的淀粉酶分泌。结论：这些结果表明CREB的毒蕈碱和β-肾上腺素激活是通过PKA途径介导的，并且CREB与腮腺腺泡细胞的蛋白质分泌有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号