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首页> 外文期刊>Parasite Immunology >The development of immune responses in Balb/c mice following inoculation with attenuated or virulent Neospora caninum tachyzoites.
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The development of immune responses in Balb/c mice following inoculation with attenuated or virulent Neospora caninum tachyzoites.

机译：减毒或强毒新孢子虫速殖子接种后，Balb / c小鼠免疫应答的发展。

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Balb/c mice were inoculated intraperitoneally (i.p.) with either 5 x 10(6) live virulent (group 1) or 5 x 10(6) live attenuated (group 2) tachyzoites, or Vero cells (group 3). Animals were killed at 0, 14, 28 and 42 days post-inoculation (p.i.), with the remaining mice receiving a lethal challenge on day 48 p.i. Serum, spleen and brain samples were collected post-mortem to examine humoral and cell-mediated immune responses as well as pathological lesions and to quantify parasite loads. On day 14 p.i. group 2 (attenuated) demonstrated statistically significant (P < 0.001) lower levels of mean morbidity and weight loss, while also showing significantly (P = 0.01) higher levels of splenocyte proliferation and IFN-gamma production (P = 0.003), compared to group 1 (virulent). Histology of brain samples showed milder lesions and a lower incidence of positive immunohistochemistry, demonstrating tachyzoites and tissue cysts, and statistically significant (P = 0.03) lower mean burdens of parasite DNA in group 2 (attenuated) compared to group 1 (virulent). All mice in group 2 were protected following challenge on day 48 p.i. whereas naive control mice succumbed to the challenge. No mice from group 1 (virulent) survived beyond day 24 p.i. so they were not included in the challenge.
机译：将Balb / c小鼠腹膜内（i.p.）接种5 x 10（6）活毒力（第1组）或5 x 10（6）减毒活菌（第2组）速殖子或Vero细胞（第3组）。接种后0、14、28和42天（p.i.）处死动物，其余小鼠在p.i 48天接受致命攻击。验尸后收集血清，脾脏和大脑样本，以检查体液和细胞介导的免疫反应以及病理性病变，并量化寄生虫负荷。下午14天与第2组相比，第2组（减弱）的平均发病率和体重减轻水平具有统计学显着性（P <0.001），同时脾细胞增殖和IFN-γ产生水平也有显着（P = 0.01）（P = 0.003） 1（有毒）。脑样本的组织学检查显示病灶较轻，免疫组织化学阳性的发生率较低，表明速殖子和组织囊肿，与组1（有毒）相比，组2（减毒）寄生虫DNA的平均平均负担（统计学上显着（P = 0.03）较低）。攻击后第48天，第2组的所有小鼠均受到保护。而幼稚的对照小鼠屈服于挑战。第1组（有毒）的小鼠在p.i 24天后都没有存活。因此它们不包含在挑战中。

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