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首页> 外文期刊>Pesticide Biochemistry and Physiology >Transgenic expression of the Aedes aegypti CYP9J28 confers pyrethroid resistance in Drosophila melanogaster. (Special Issue: Molecular approaches to pest control, toxicology and resistance: dedicated to Professor Elvins Yuill Spencer, Ph.D. Editor-in-Chief, Pesticide Biochemistry and Physiology (1978-1990).)
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Transgenic expression of the Aedes aegypti CYP9J28 confers pyrethroid resistance in Drosophila melanogaster. (Special Issue: Molecular approaches to pest control, toxicology and resistance: dedicated to Professor Elvins Yuill Spencer, Ph.D. Editor-in-Chief, Pesticide Biochemistry and Physiology (1978-1990).)

机译：埃及伊蚊CYP9J28的转基因表达在果蝇中赋予拟除虫菊酯抗性。 （特刊：害虫控制，毒理学和抗药性的分子方法：专为埃尔文斯·尤尔·斯宾塞教授，农药生物化学和生理学首席编辑（1978-1990年）。）

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摘要

The emergence and spread of insecticide resistance in mosquitoes, such as the major vector of dengue and yellow fever Aedes aegypti, is a major public health problem. A number of studies have been conducted to-date aiming to identify specific molecular changes that are associated with the phenotype, in an attempt to use this knowledge to control the development and spread of resistant populations. Several approaches, ranging from classical techniques to high-throughput transcriptomics, have revealed a large number of genes which are associated with the trait. However, the development of functional tools for the validation of the possible role of those genes (if any) in mosquito resistance has not kept similar pace. Here, we report the use of Drosophila melanogaster as a model for establishing the role of A. aegypti cytochrome P450s. We showed that the transgenic expression of the CYP9J28 a gene which is upregulated in pyrethroid resistant A. aegypti mosquitoes and encodes an enzyme capable of metabolizing pyrethroids, in D. melanogaster confers significant levels of pyrethroid resistance. Our study provides solid functional in vivo evidence for the role of the AaegCYP9J28 in pyrethroid resistance and shows for the first time that ectopic expression of mosquito genes in D. melanogaster can be a robust approach for validating candidate resistance genes. Further potential applications of the approach are discussed.
机译：诸如登革热和黄热埃及伊蚊的主要媒介等蚊子中抗药性的出现和传播是一个重大的公共卫生问题。迄今为止已经进行了许多研究，旨在鉴定与表型有关的特定分子变化，以试图利用该知识来控制抗性种群的发展和传播。从经典技术到高通量转录组学，几种方法揭示了与该性状相关的大量基因。但是，用于验证那些基因（如果有）在蚊子抗药性中可能作用的功能性工具的开发并没有保持类似的速度。在这里，我们报告果蝇果蝇的使用，以建立埃及埃及嗜血杆菌细胞色素P450的作用模型。我们表明CYP9J28一个基因的转基因表达在拟除虫菊酯抗性埃及埃及蚊中被上调，并且编码一种能够代谢拟除虫菊酯的酶，在黑腹果蝇中具有显着水平的拟除虫菊酯抗性。我们的研究为AaegCYP9J28在拟除虫菊酯抗药性中的作用提供了可靠的体内功能证据，并首次表明异位表达蚊子在D. melanogaster中可以成为验证候选抗药性基因的可靠方法。讨论了该方法的其他潜在应用。

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