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首页> 外文期刊>Pharmaceutical development and technology >Collagen loaded nano-sized surfactant based dispersion for topical application: formulation development, characterization and safety study
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Collagen loaded nano-sized surfactant based dispersion for topical application: formulation development, characterization and safety study

机译：基于胶原蛋白的纳米表面活性剂分散体，用于局部应用：制剂开发，表征和安全性研究

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摘要

Collagen, a high molecular weight, hydrophilic and highly abundant protein is known to have anti-ageing, anti-wrinkle, anti-acne, anti-scar and wound healing properties. High molecular weight and hydrophilic nature hinder its effective topical delivery. So, the objective of present study was to develop effective topical nano-surfactant dispersion (NSD) for collagen delivery. NSD was prepared from sorbitan monostearate (Span60) and cholesterol using ethanol injection method followed by probe sonication. NSD was characterized for entrapment efficiency (%EE), size and size distribution (Z-avg and polydispersity index (PDI)), shape, zeta-potential (zeta), in vitro drug release, skin hydration and skin irritation test and histopathological examination. Optimized NSD (NSD3) had % EE, z-avg, PDI and zeta-potential of 77.56% +/- 1.09%, 158.1 +/- 2.31 nm, 0.211 and -17.2 +/- 0.64 mV, respectively. In in vivo skin hydration test, NSD treatment showed nearly 2.5-fold and 3-fold increase in the thickness of stratum corneum (SC) as compared to the collagen gel treated and untreated skin, respectively. The mean scores of skin irritation test in two animal species, rats and rabbits, were found to be 1.42 +/- 1.01 and 1.71 +/- 0.29, respectively, indicating the non-irritant nature of collagen loaded NSD. Histopathology of the skin after application of developed NSD showed non-significant changes in skin anatomy indicating its safe nature.
机译：胶原蛋白是一种高分子量，亲水性和高度丰富的蛋白质，已知具有抗衰老，抗皱，抗痤疮，抗疤痕和伤口愈合的特性。高分子量和亲水性阻碍了其有效的局部递送。因此，本研究的目的是开发有效的局部纳米表面活性剂分散液（NSD）用于胶原蛋白的输送。 NSD由脱水山梨糖醇单硬脂酸酯（Span60）和胆固醇使用乙醇注射法，然后进行探针超声处理制备。 NSD的特征在于包封率（％EE），尺寸和尺寸分布（Z-平均和多分散性指数（PDI）），形状，ζ-电势（zeta），体外药物释放，皮肤水化和皮肤刺激性测试以及组织病理学检查。优化的NSD（NSD3）的％EE，z-avg，PDI和zeta电位分别为77.56％+/- 1.09％，158.1 +/- 2.31 nm，0.211和-17.2 +/- 0.64 mV。在体内皮肤水合测试中，与胶原蛋白凝胶处理的皮肤和未处理的皮肤相比，NSD处理的角质层（SC）厚度分别增加了近2.5倍和3倍。发现在两种动物物种（大鼠和兔子）中皮肤刺激试验的平均得分分别为1.42 +/- 1.01和1.71 +/- 0.29，表明胶原蛋白负载的NSD的无刺激性。应用发达的NSD后皮肤的组织病理学显示皮肤解剖结构无明显变化，表明其安全性。

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