ALKALINE PROTEASE AND LYSOZYME IMMOBILIZED IN MODIFIED POLY(N-VINYLPYRROLIDONE)

I.I. Romanovskaya; S. S. Dekina; 1; I.I. Pashkin

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ALKALINE PROTEASE AND LYSOZYME IMMOBILIZED IN MODIFIED POLY(N-VINYLPYRROLIDONE)

机译：固定化改性聚（N-乙烯基吡咯烷酮）中的碱性蛋白酶和溶菌酶

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Alkaline protease from Bacillus subtilis and lysozyme from chicken egg white have been immobilized for the first time in poly(N-vinylpyrrolidone) modified by polysilicic acid sol. Elastic polymeric hydrogels that are insoluble under physiological conditions are obtained and provide potential wound-healing coatings with high retention of lytic and proteolytic activities (80 - 100%) and prolonged action (24 h) and complex preparations with proteolytic and 1.7-fold increased lytic activity. It is established that immobilized alkaline protease and lysozyme are stable under conditions modeling the wound pH level during storage for one year and upon sterilization by y- irradiation.

机译：枯草芽孢杆菌的碱性蛋白酶和鸡蛋白的溶菌酶已首次固定在由聚硅酸溶胶改性的聚（N-乙烯基吡咯烷酮）中。获得了在生理条件下不溶的弹性聚合物水凝胶，并提供了潜在的伤口愈合涂层，具有高的溶解和蛋白水解活性（80-100％）和延长的作用（24小时），以及复杂的制剂，具有水解和蛋白水解的1.7倍活动。已经确定，固定的碱性蛋白酶和溶菌酶在模拟在储存一年中和通过γ射线灭菌后的伤口pH水平的条件下是稳定的。

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《Pharmaceutical Chemistry Journal》 |2009年第11期|共4页
作者
I.I. Romanovskaya; S. S. Dekina; 1; I.I. Pashkin;
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正文语种 eng
中图分类药学;
关键词
alkaline protease; lysozyme; immobilization; poly(N-vinylpyrrolidone).;

机译：碱性蛋白酶;溶菌酶;固定化;聚（N-乙烯基吡咯烷酮）;

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专利

1. ALKALINE PROTEASE AND LYSOZYME IMMOBILIZED IN MODIFIED POLY(N-VINYLPYRROLIDONE) [J] . I.I. Romanovskaya, S. S. Dekina, 1, Pharmaceutical Chemistry Journal . 2009,第11期

机译：固定化改性聚（N-乙烯基吡咯烷酮）中的碱性蛋白酶和溶菌酶
2. In Situ Immobilization of Alkaline Protease during Inverse Suspension Polymerization of Polyacrylamide and Poly(acrylamide-co-methacrylic acid) Hydrogel Beads [J] . S.Kiatkamjornwong, N.Siwarungson, NGANBUNSRI A Journal of Applied Polymer Science . 1999,第11期

机译：聚丙烯酰胺和聚丙烯酰胺-共-甲基丙烯酸水凝胶珠的反向悬浮聚合过程中碱性蛋白酶的原位固定
3. Poly(N-vinylpyrrolidone)-modified poly(dimethylsiloxane) elastomers as anti-biofouling materials [J] . Wu Z., Tong W., Jiang W., Colloids and Surfaces, B. Biointerfaces . 2012,第Null期

机译：聚（N-乙烯基吡咯烷酮）改性的聚（二甲基硅氧烷）弹性体作为防污垢材料
4. Poly(N-vinylpyrrolidone)-Modified Poly(dimethylsiloxane) Elastomers as Anti-Biofouling Materials [C] . Zhaoqiang Wu, Weifang Tong, Xiaoli Liu, World biomaterials congress . 2012

机译：聚（N-乙烯基吡咯烷酮）改性的聚（二甲基硅氧烷）弹性体作为防污垢材料
5. Part I. An investigation of the mechanism of interaction between poly(n-vinylpyrrolidone) and phenols. Part II. An investigation into a more efficacious imaging agent. [D] . Johnson, Helen Jane. 1994

机译：第一部分。聚（n-乙烯基吡咯烷酮）与酚之间相互作用机理的研究。第二部分对更有效的成像剂的研究。
6. Kinetic and thermodynamic properties of purified alkaline protease from Bacillus pumilus Y7 and non‐covalent immobilization to poly(vinylimidazole)/clay hydrogel [O] . Yonca Avcı Duman, Nalan Tekin 2020

机译：短小芽孢杆菌Y7纯化碱性蛋白酶的动力学和热力学性质以及非共价固定化聚乙烯基咪唑/粘土水凝胶的动力学和热力学性质
7. BOD biosensor based on the yeast Debaryomyces hansenii immobilized in poly(vinyl alcohol) modified by N-vinylpyrrolidone [O] . Arlyapov V.A., Yudina N.Yu., Asulyan L.D., 2013

机译：基于固定在N-乙烯基吡咯烷酮改性的聚乙烯醇上的汉逊酵母（Debaryomyces hansenii）酵母的BOD生物传感器

ALKALINE PROTEASE AND LYSOZYME IMMOBILIZED IN MODIFIED POLY(N-VINYLPYRROLIDONE)

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