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首页> 外文期刊>PharmaChem >Efficient infrared (IR) heating method for solid-phase peptide synthesis on the Tribute? and Symphony X? peptide synthesizers
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Efficient infrared (IR) heating method for solid-phase peptide synthesis on the Tribute? and Symphony X? peptide synthesizers

机译：在Tribute上进行固相肽合成的高效红外（IR）加热方法？和交响乐X？肽合成仪

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摘要

Many parameters may be optimized in the solid-phase synthesis of a peptide, including the reaction times, activator type, reagent excess and concentration, solvent, and resin loading. For the synthesis of long or difficult peptides, residues like pseudoprolines that disrupt secondary/ structure and aggregation have proven to be effective (1-3). The application of heat, using oil baths, heating elements, or microwaves, has emerged as an additional tool for peptide synthesis (4). Microwave heating in particular has become popular in recent years, due to the speed with which small volumes can be raised to elevated temperatures. While there has been some speculation regarding the existence of a non-thermal "microwave effect", a thorough series of experiments examining the synthesis of both peptides (5) and small molecules (6,7) has demonstrated that observed enhancements in purity can be explained by purely thermal effects. Apparent discrepancies in the results between microwave and non-microwave heating could be eliminated by careful temperature monitoring and control.
机译：在肽的固相合成中可以优化许多参数，包括反应时间，活化剂类型，试剂过量和浓度，溶剂和树脂负载量。对于长肽或困难肽的合成，已证明能破坏二级/结构和聚集的伪脯氨酸等残基是有效的（1-3）。使用油浴，加热元件或微波进行加热已成为肽合成的另一种工具（4）。近年来，由于可以将小体积升高到高温的速度，特别是微波加热已变得很流行。尽管有一些关于非热“微波效应”存在的推测，但一系列彻底研究了肽（5）和小分子（6,7）合成的实验表明，观察到的纯度提高可以纯粹由热效应解释。通过仔细的温度监测和控制，可以消除微波和非微波加热结果之间的明显差异。

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