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首页> 外文期刊>Sabrao Journal of Breeding and Genetics >EVALUATION OF RESISTANCE IN Brassica rapa TO DARK POD SPOT (Alternaria brassicicola) USING THE IN VITRO DETACHED POD ASSAY
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EVALUATION OF RESISTANCE IN Brassica rapa TO DARK POD SPOT (Alternaria brassicicola) USING THE IN VITRO DETACHED POD ASSAY

机译：利用离体荚果分析法评价小白菜对暗斑病的抗性

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摘要

Alternaria brassicicola is a common virulent seed-borne fungal pathogen globally found in Brassica crops. Various factors for inoculation of detached pods were studied using several Brassica rapa genotypes to identify resistance in the pod setting stage of B. rapa cultivars. Optimal conditions for pod inoculation were (1) inoculum concentration of 5 x 10(3) conidia/ml, (2) incubation temperature of 20-25 degrees C, and (3) a 48-h waiting period post inoculation using 25-day-old pods. A pod screening test using 43 cultivars of B. rapa effectively distinguished various levels of resistance between cultivars. As a result, we identified cultivars viz 'Yajima kabu', 'Saishin' and 'Shimofusa kabu' as resistant. The resistant B. rapa cultivars are useful not only for B. rapa breeding, but also for breeding programs for other Brassica species, such as B. napus, which is derived from B. rapa and B. oleracea and has little genetic variation due to the limited size of the descendent population.
机译：交链孢霉是芸苔属作物中普遍发现的一种常见的有毒种子传播真菌病原体。研究了使用几种甘蓝型油菜的基因型研究分离豆荚接种的各种因素，以鉴定B. rapa品种的豆荚定型阶段的抗性。荚果接种的最佳条件是：（1）接种物浓度为5 x 10（3）分生孢子/ ml，（2）孵化温度为20-25摄氏度，（3）接种后等待48小时（使用25天）老豆荚。使用43个B. rapa品种的荚果筛选试验可以有效地区分不同品种之间的抗性水平。结果，我们鉴定出'Yajima kabu'，'Saishin'和'Shimofusa kabu'两个品种具有抗性。抗性的B. rapa品种不仅可用于B. rapa育种，而且还可用于其他芸苔属物种（例如B. napus）的育种程序，这些物种来源于B. rapa和B. oleracea，并且由于子孙后代的数量有限。

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