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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Delayed stress-induced antinociceptive effect of nitric oxide synthase inhibition in the dentate gyrus of rats.
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Delayed stress-induced antinociceptive effect of nitric oxide synthase inhibition in the dentate gyrus of rats.

机译：延迟应激诱导的大鼠齿状回中一氧化氮合酶的镇痛作用。

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摘要

Stimulation of the hippocampal formation can modulate nociceptive mechanisms, whereas painful stimuli can activate this structure. Stress exposure can produce plastic changes in the hippocampus. Nitric oxide (NO) is an important neuroregulatory agent present in the hippocampus. The objective of the present study was to investigate the effects of intrahippocampal administration of N(omega)-nitro-L-arginine methyl ester hydrochloride (L-NAME), an inhibitor of NO synthase (NOS), on nociceptive processes in stressed and nonstressed rats. Male Wistar rats (n=6-11/group) received unilateral microinjection of L-NAME (50-300 nmol/0.2 &mgr;l) into the dentate gyrus (DG) of the dorsal hippocampus. Immediately after the injection tail-flick reflex latency was measured. Stressed animals were submitted to 2 h of restraint and tested immediately or 1, 2, 5 or 10 days later. L-NAME failed to modify nociception in nonstressed rats. However, 5 days after, restraint L-NAME, at all doses tested, produced an antinociceptive effect (ANOVA, P<.05). The dose-response curve had an inverted U shape. L-NAME antinociceptive effect was antagonized by previous treatment with L-arginine (150 nmol/0.2 &mgr;l, P<.05). The results suggest that the modulation of nociceptive processes by NO in the dorsal hippocampus is dependent on previous stress exposure and on poststress interval.
机译：海马结构的刺激可调节伤害感受机制，而疼痛刺激可激活该结构。压力暴露会在海马体中产生塑性变化。一氧化氮（NO）是海马中存在的一种重要的神经调节剂。本研究的目的是研究海马内给予NO合酶（NOS）抑制剂N（ω）-硝基-L-精氨酸甲酯盐酸盐（L-NAME）对应激和非应激过程中伤害感受的影响大鼠。雄性Wistar大鼠（n ＝ 6-11 /组）被单侧显微注射L-NAME（50-300nmol /0.2μl）到背侧海马的齿状回（DG）中。注射后立即测量甩尾反射潜伏期。使受压动物束缚2小时，并立即或在1、2、5或10天后进行测试。 L-NAME无法改变非应激大鼠的伤害感受。然而，在5天后，在所有测试剂量下，克制L-NAME均会产生抗伤害感作用（ANOVA，P <.05）。剂量反应曲线呈倒U形。通过先前用L-精氨酸（150nmol /0.2μl，P ＜0.05）的治疗来拮抗L-NAME抗伤害感受作用。结果表明，背海马体中NO对伤害感受过程的调节取决于先前的应激暴露和应激后间隔。

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