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Estrous cycle and food availability affect feeding induced by amygdala 5-HT receptor blockade.

机译:发情周期和食物供应量会影响杏仁核5-HT受体阻滞诱导的进食。

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We have recently reported that bilateral infusions of the 5-HT receptor antagonist metergoline (MET) into the posterior basolateral amygdala (pBLA) elicit feeding in female rats tested at mid-light cycle. The present study was performed to determine whether (1) testing at two different phases of the estrous cycle, and/or (2) the palatability of the food might modify this effect. Subjects were 18 adult females with bilateral pBLA cannulae. Following familiarization with Froot Loops cereal, a within-subjects design tested all animals for 1- and 2-h food intake under 2 Drug (0.3 nmol MET vs. Vehicle), 2 Estrous Cycle (diestrus vs. estrus) and 2 Food (lab chow vs. Froot Loops) conditions. Rats weighed more at diestrus than at proestrus (P<.05) or estrus (P<.005). Multivariate analyses of variance (MANOVAs) revealed a preference for Froot Loops over lab chow (P<.0001). MET increased feeding regardless of food type (P<.0001). Rats ate more Froot Loops (P<.01), but not lab chow, at diestrus vs. estrus. A three-way interaction (P<.05) showed rats ate more during the first hour in estrus than in diestrus to lab chow but not Froot Loops. These data suggest pBLA MET differentially affects feeding over the estrous cycle depending on the palatability of food available.
机译:我们最近有报道说,在中期光照周期测试的雌性大鼠中,向后基底外侧杏仁核(pBLA)两侧注入5-HT受体拮抗剂美特古琳(MET)会引起进食。进行本研究是为了确定(1)在发情周期的两个不同阶段进行测试,和/或(2)食物的适口性是否可以改变这种效果。受试者为18名成年女性,双侧pBLA套管。熟悉Froot Loops谷物后,受试者内部设计在2种药物(0.3 nmol MET与媒介物),2个发情周期(二种谷物与发情期)和2种食物(实验室)下测试了所有动物的1小时和2小时食物摄入量chow vs.Froot Loops)条件。大鼠发情期的体重比发情期(P <.05)或发情期(P <.005)的多。多变量方差分析(MANOVAs)显示,Froot Loops优于实验室食物(P <.0001)。无论食物类型如何,MET都会增加饲喂量(P <.0001)。在发情期与发情期之间,大鼠吃了更多的Froot Loops(P <.01),但没有实验性食物。三向交互作用(P <.05)显示,在发情的第一小时内,大鼠对实验食物的摄入量要比在发情期的摄入量多,但Froot Loops的摄入量却不多。这些数据表明,pBLA MET取决于动荡食物的适口性,在发情周期内对进食有不同的影响。

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