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首页> 外文期刊>Pharmacology and Toxicology: An International Journal >In Vitro Induction of Differentiation by Ginsenoside Rh2 in SMMC-7721 Hepatocarcinoma Cell Line.
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In Vitro Induction of Differentiation by Ginsenoside Rh2 in SMMC-7721 Hepatocarcinoma Cell Line.

机译：人参皂甙Rh2在SMMC-7721肝癌细胞系中体外诱导分化。

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摘要

The aim of this study was to investigate the effects of ginsenoside Rh2 (G-Rh2) on differentiation of SMMC-7721 hepatocarcinoma cell line in culture. We studied G-Rh2-induced differentiation of SMMC-7721 cells through cell proliferation, cell morphology, ultrastructure, cell cycle, cell function and metabolism. The proliferation of treated cells was inhibited, the morphology and ultrastructure seemed normal, the secretory amount and expression of alpha-foetoprotein, and the specific activity of gamma-glutamyl transpeptidase, and heat-resistant alkaline phosphatase were all significantly decreased, the secretory amount of albumin and alkaline phosphatase activity were remarkably increased, and the cell was arrested at the G1/G0 phase. Furthermore, G-Rh2 induced elevated expression of the cyclin-dependent kinase inhibitor p21WAF1 and p16INK4a, and declined expressions of cyclin D1 and cyclin E. In addition, G-Rh2 almost completely inhibited telomerase activity, as measured by polymerase chain reaction-based telomeric repeat amplification protocol coupled with enzyme-linked immune sorbent assay, and human telomerase reverse transcriptase mRNA. Based on these data, it is suggested that G-Rh2 could induce cell differentiation tending to normal and effectively reduce telomerase activity with affecting transcription levels of human telomerase reverse transcriptase, paralleling the induction of cell differentiation.
机译：这项研究的目的是研究人参皂甙Rh2（G-Rh2）对培养物中SMMC-7721肝癌细胞系分化的影响。我们通过细胞增殖，细胞形态，超微结构，细胞周期，细胞功能和代谢研究了G-Rh2诱导的SMMC-7721细胞分化。处理细胞的增殖受到抑制，形态和超微结构似乎正常，α-甲胎蛋白的分泌量和表达，γ-谷氨酰转肽酶和耐热碱性磷酸酶的比活性均显着降低，白蛋白和碱性磷酸酶活性显着增加，细胞停滞在G1 / G0期。此外，G-Rh2诱导细胞周期蛋白依赖性激酶抑制剂p21WAF1和p16INK4a的表达升高，而细胞周期蛋白D1和细胞周期蛋白E的表达下降。此外，通过基于聚合酶链反应的端粒测量，G-Rh2几乎完全抑制了端粒酶活性。重复扩增方案，再加上酶联免疫吸附测定和人类端粒酶逆转录酶mRNA。基于这些数据，表明G-Rh2可以诱导趋于正常的细胞分化并且有效地降低端粒酶活性，同时影响人端粒酶逆转录酶的转录水平，同时诱导细胞分化。

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