• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pharmacology and Toxicology: An International Journal >Lindane (gamma-hexachlorocyclohexane) induces internal Ca2+ release and capacitative Ca2+ entry in Madin-Darby canine kidney cells.
【24h】

Lindane (gamma-hexachlorocyclohexane) induces internal Ca2+ release and capacitative Ca2+ entry in Madin-Darby canine kidney cells.

机译：林丹（γ-六氯环己烷）在Madin-Darby犬肾细胞中诱导内部Ca2 +释放和功能性Ca2 +进入。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effect of lindane (gamma-hexachlorocyclohexane), an organochlorine pesticide, on Ca2+ mobilization in Madin-Darby canine kidney cells was examined by fluorimetry using fura-2 as a Ca2+ indicator. Lindane (5-200 microM) increased [Ca2+]i concentration-dependently. The [Ca2+]i signal comprised an immediate initial rise followed by a persistent phase. Ca2+ removal inhibited the [Ca2+]i signal by reducing both the initial rise and the sustained phase. This implies lindane-triggered Ca2+ influx and Ca2+ release. In Ca2+ -free medium, 0.15 mM lindane increased [Ca2+]i after pretreatment with carbonylcyanide m-chlorophenylhydrazone (CCCP, 2 microM), a mitochondrial uncoupler, and two endoplasmic reticulum Ca2+ pump inhibitors, thapsigargin and cyclopiazonic acid. Conversely, pretreatment with lindane abolished CCCP- and thapsigargin-induced Ca2+ release. This suggests that 0.15 mM lindane released Ca2+ from the endoplasmic reticulum, mitochondria and other stores. La3+ (1 mM) partly inhibited 0.1 mM lindane-induced [Ca2+]i increase, confirming that lindane induced Ca2+ influx. Addition of 3 mM Ca2+ increased [Ca2+]i after pretreatment with 0.15 mM lindane for 750 sec. in Ca2+ -free medium, which indicates lindane-induced capacitative Ca2+ entry. Lindane (0.15 mM)-induced Ca2+ release was not reduced by inhibiting phospholipase C with 2 microM U73122, but was inhibited by 70% by the phospholipase A2 inhibitor aristolochic acid (40 microM).
机译：使用fura-2作为Ca2 +指示剂，通过荧光法检测了林丹（γ-六氯环己烷）（一种有机氯农药）对Madin-Darby犬肾细胞中Ca2 +动员的影响。林丹（5-20​​0 microM）依赖性地增加[Ca2 +] i的浓度。 [Ca 2+] i信号包括立即初始上升，然后是持续阶段。 Ca 2+的去除通过减少初始上升和持续相抑制了[Ca2 +] i信号。这意味着林丹触发了Ca2 +内流和Ca2 +释放。在不含Ca2 +的培养基中，用羰基氰间氯苯hydr（CCCP，2 microM），线粒体解偶联剂和两种内质网Ca2 +泵抑制剂，毒胡萝卜素和环吡嗪酸预处理后，0.15 mM林丹增加[Ca2 +] i。相反，用林丹进行的预处理废除了CCCP和毒胡萝卜素诱导的Ca2 +释放。这表明0.15 mM林丹从内质网，线粒体和其他存储释放Ca2 +。 La3 +（1 mM）部分抑制了0.1 mM林丹诱导的[Ca2 +] i增加，证实了林丹诱导的Ca2 +流入。用0.15 mM林丹预处理750秒后，添加3 mM Ca2 +会增加[Ca2 +] i。不含Ca2 +的培养基中，表明林丹诱导的Ca2 +进入容性。用2 microM U73122抑制磷脂酶C并不会降低林丹（0.15 mM）诱导的Ca2 +释放，但是磷脂酶A2抑制剂马兜铃酸（40 microM）可以抑制林丹70％。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号