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首页> 外文期刊>Pharmacology and Toxicology: An International Journal >Adjuvant effect of di-n-butyl-, di-n-octyl-, di-iso-nonyl- and di-iso-decyl phthalate in a subcutaneous injection model using BALB/c mice.
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Adjuvant effect of di-n-butyl-, di-n-octyl-, di-iso-nonyl- and di-iso-decyl phthalate in a subcutaneous injection model using BALB/c mice.

机译：在使用BALB / c小鼠的皮下注射模型中，邻苯二甲酸二正丁酯，邻苯二甲酸正辛酯，邻苯二甲酸二异壬酯和邻苯二甲酸二异癸酯的佐剂作用。

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摘要

During the last decades, the prevalence of the allergic airway diseases, asthma and rhinitis, has increased world-wide. Introduction of environmental chemicals with adjuvant effect may play a role in this increase. In the present study, the adjuvant effects of di-n-butyl-, di-n-octyl-, di-iso-nonyl- and di-iso-decyl phthalate are studied in a screening model. Ovalbumin, used as the model antigen, was injected subcutaneously in the neck region of BALB/cJ mice with the selected phthalate in concentrations from 2-2000 microg/ml. Additionally, the mice were boosted once or twice with ovalbumin alone. Immunization with ovalbumin alone, the ovalbumin control group, served as the baseline for antibody production, whereas aluminium hydroxide served as the positive control. The levels of ovalbumin-specific IgE, IgG1 and IgG2a antibodies in sera were determined. Adjuvant effect was accepted to be present if a statistical increase in antibody production occurred in a test group as compared to an ovalbumin controlgroup together with the fulfillment of dose-response relationships. Adjuvant effect varied strongly between the phthalates investigated. Phthalates with 8 or 9 carbon atoms in the alkyl side chains were the stronger adjuvants whereas phthalates with shorter or longer alkyl side chains possessed less adjuvant activity. Adjuvant effects were apparent either from the IgE or the IgG1 response or both, whereas no effect was seen on the IgG2a response. Additional studies with airborne exposure are required to establish whether the hazards also result in a significant risk for the development of allergy in man.
机译：在过去的几十年中，过敏性气道疾病，哮喘和鼻炎的流行在全世界范围内都在增加。具有辅助作用的环境化学品的引入可能在这种增加中起作用。在本研究中，在筛选模型中研究了邻苯二甲酸二正丁酯，邻苯二甲酸二正辛酯，邻苯二甲酸二异壬酯和邻苯二甲酸二异癸酯的佐剂作用。将用作模型抗原的卵清蛋白皮下注射浓度为2-2000 microg / ml的所选邻苯二甲酸酯到BALB / cJ小鼠的颈部。另外，仅用卵清蛋白对小鼠加强一次或两次。卵清蛋白对照组仅以卵清蛋白免疫，作为抗体产生的基线，而氢氧化铝为阳性对照组。测定血清中卵清蛋白特异性IgE，IgG1和IgG2a抗体的水平。如果与卵清蛋白对照组相比，与卵白蛋白对照组相比，试验组中抗体产生的统计增加发生，则认为存在佐剂作用。所研究的邻苯二甲酸酯之间的佐剂效果差异很大。在烷基侧链中具有8或9个碳原子的邻苯二甲酸酯是更强的佐剂，而具有较短或较长烷基侧链的邻苯二甲酸酯具有较少的佐剂活性。从IgE或IgG1响应或两者都有明显的佐剂作用，而对IgG2a响应没有作用。需要进行更多的空气传播研究，以确定危害是否也对人类过敏发展产生重大风险。

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