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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >The relationship of oral chlorpyrifos effects on behavior, cholinesterase inhibition, and muscarinic receptor density in rat.
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The relationship of oral chlorpyrifos effects on behavior, cholinesterase inhibition, and muscarinic receptor density in rat.

机译：口服毒死rif对大鼠行为，胆碱酯酶抑制和毒蕈碱受体密度的影响。

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摘要

Behavioral changes and tissue cholinesterase (ChE) inhibition were examined in animals treated with the commonly used insecticide chlorpyrifos. Adult male rats were dosed by gavage with 0, 10, 30, 60, or 100 mg/kg chlorpyrifos. Rats (n = 20/dose group) were evaluated using a functional observational battery (FOB) and an automated measure of motor activity. All rats were tested the day before dosing and at 3.5 h (the time of peak effect) after dosing; half of these (n = 10/dose) were sacrificed immediately after testing for tissue collection. The remaining rats were tested again at 24 h, followed by sacrifice. The following tissues were collected from each animal: half brain, individual brain areas from the other half of the brain (frontal cortex, hippocampus, striatum, hypothalamus, cerebellum, pons/medulla), retina, liver, heart, diaphragm, quadriceps femoris muscle, and blood (separated into whole blood, plasma, and erythrocytes). ChE activity was measured in all tissues, and muscarinic receptor density was assessed as quinuclidinyl benzilate (QNB) binding in all brain regions, heart, and retina. The lowest dose produced no behavioral effects but did produce significant ChE inhibition in most tissues at 3.5 h. Higher doses produced more ChE inhibition and cholinergic signs of toxicity. Partial recovery from behavioral effects was evident at 24 h, with little or no corresponding recovery of ChE activity. Apparent downregulation of muscarinic receptor density was noted only in striatum and pons/medulla of rats treated with the highest dose of chlorpyrifos. Correlations for behavioral and biochemical effects were generally poor because: a) the low-dose effects on ChE inhibition were not reflected in behavioral signs, and b) behavioral signs showed recovery at 24 h, whereas ChE activity did not. Examination of data for individual rats indicated that > 60% of brain ChE inhibition was reached before neurobehavioral effects were evident.
机译：在用常用杀虫剂毒死rif治疗的动物中检查了行为变化和组织胆碱酯酶（ChE）抑制。用管饲法给成年雄性大鼠施用0、10、30、60或100 mg / kg毒死rif。使用功能性观察电池（FOB）和运动活动的自动测量对大鼠（n = 20 /剂量组）进行评估。在给药前一天和给药后3.5小时（达到峰值的时间）测试所有大鼠。测试组织收集后立即将其中一半（n = 10 /剂量）处死。在24小时再次测试其余的大鼠，然后处死。从每只动物收集以下组织：半个大脑，大脑另一半的各个大脑区域（额叶皮层，海马，纹状体，下丘脑，小脑，脑桥/延髓），视网膜，肝脏，心脏，diaphragm肌，股四头肌和血液（分为全血，血浆和红细胞）。在所有组织中测量ChE活性，并以在所有脑区域，心脏和视网膜中的苯并喹啉基苄基酸酯（QNB）结合来评估毒蕈碱受体密度。最低剂量没有产生任何行为影响，但在3.5 h的大多数组织中却产生了显着的ChE抑制作用。更高的剂量产生更多的ChE抑制作用和胆碱能中毒迹象。在24小时内，行为行为可部分恢复，而很少或没有相应的ChE活性恢复。仅在用最高剂量毒死rif治疗的大鼠的纹状体和脑桥/延髓中才注意到毒蕈碱受体密度的明显下调。行为和生化作用的相关性通常较差，因为：a）对ChE抑制作用的低剂量效应未反映在行为征兆上，b）行为征兆在24 h时恢复，而ChE活性未见。对单个大鼠的数据检查表明，在明显的神经行为作用之前，已经达到了60％以上的脑ChE抑制作用。

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