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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Microdialysis of GABA and glutamate: Analysis, interpretation and comparison with microsensors.
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Microdialysis of GABA and glutamate: Analysis, interpretation and comparison with microsensors.

机译：GABA和谷氨酸的微透析：与微传感器的分析，解释和比较。

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摘要

GABA and glutamate sampled from the brain by microdialysis do not always fulfill the classic criteria for exocytotic release. In this regard the origin (neuronal vs. astroglial, synaptic vs. extrasynaptic) of glutamate and GABA collected by microdialysis as well as in the ECF itself, is still a matter of debate. In this overview microdialysis of GABA and glutamate and the use of microsensors to detect extracellular glutamate are compared and discussed. During basal conditions glutamate in microdialysates is mainly derived from non-synaptic sources. Indeed recently several sources of astrocytic glutamate release have been described, including glutamate derived from gliotransmission. However during conditions of (chemical, electrical or behavioral) stimulation a significant part of glutamate might be derived from neurotransmission. Interestingly accumulating evidence suggests that glutamate determined by microsensors is more likely to reflect basal synaptic events. This would mean that microdialysis and microsensors are complementary methods to study extracellular glutamate. Regarding GABA we concluded that the chromatographic conditions for the separation of this transmitter from other amino acid-derivatives are extremely critical. Optimal conditions to detect GABA in microdialysis samples - at least in our laboratory - include a retention time of approximately 60 min and a careful control of the pH of the mobile phase. Under these conditions it appears that 50-70% of GABA in dialysates is derived from neurotransmission.
机译：通过微透析从大脑中采样的GABA和谷氨酸并不总是满足经典的胞吐释放标准。在这方面，通过微透析以及ECF本身收集的谷氨酸和GABA的起源（神经元还是星形胶质细胞，突触还是突触外）仍然是一个有争议的问题。在本概述中，对GABA和谷氨酸的微透析以及使用微传感器检测细胞外谷氨酸的使用进行了比较和讨论。在基础条件下，微量透析液中的谷氨酸主要来自非突触来源。实际上，最近已经描述了星形细胞谷氨酸释放的几种来源，包括源自神经胶质传递的谷氨酸。但是，在（化学，电或行为）刺激条件下，谷氨酸的很大一部分可能来自神经传递。有趣的是，越来越多的证据表明，由微传感器测定的谷氨酸更可能反映基础突触事件。这意味着微透析和微传感器是研究细胞外谷氨酸的补充方法。关于GABA，我们得出结论，将该递质与其他氨基酸衍生物分离的色谱条件非常关键。至少在我们的实验室中，检测微透析样品中GABA的最佳条件包括保留时间约60分钟，并仔细控制流动相的pH。在这些条件下，透析液中GABA的50-70％似乎来自神经传递。

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