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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Intrathecal administration of roscovitine attenuates cancer pain and inhibits the expression of NMDA receptor 2B subunit mRNA
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Intrathecal administration of roscovitine attenuates cancer pain and inhibits the expression of NMDA receptor 2B subunit mRNA

机译：鞘内注射roscovitine可减轻癌症疼痛并抑制NMDA受体2B亚基mRNA的表达

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摘要

Cancer pain is one of the most severe chronic pains. The mechanisms underlying cancer pain are still unclear. Because of the pain-relieving effects of Cdk5 (Cyclin-dependent kinase 5) antagonist roscovitine in inflammation pain models, we tested whether roscovitine would induce antihyperalgesia in cancer pain. Our previous study showed that the NR2B (N-methyl-d-aspartate receptor 2B) in the spinal cord participates in bone cancer pain in mice. In this study, we used a mouse model of bone cancer pain to investigate whether roscovitine could attenuate bone cancer pain by regulating the expression level of NR2B mRNA in spinal cord. C3H/HeJ mice were inoculated into the intramedullary space of the right femur with Osteosarcoma cells to induce ongoing bone cancer pain behaviors. At day 14 after operation, inoculation of Osteosarcoma cells significantly enhanced mechanical allodynia and thermal hyperalgesia, which was attenuated by intrathecal administration of different doses of roscovitine. Correlated with the pain behaviors changes, RT-PCR experiments in our study revealed that there was a marked increase in the expression of NR2B mRNA in spinal cord after operation, which was attenuated by intrathecal administration of roscovitine. These results suggest that roscovitine may be a useful adjunct therapy for bone cancer pain, and NR2B in spinal cord may participate in this effect.
机译：癌症疼痛是最严重的慢性疼痛之一。癌症疼痛的潜在机制仍不清楚。由于Cdk5（细胞周期蛋白依赖性激酶5）拮抗剂roscovitine在炎症性疼痛模型中具有止痛作用，因此我们测试了roscovitine是否会在癌症疼痛中诱导抗痛觉过敏。我们以前的研究表明，脊髓中的NR2B（N-甲基-d-天冬氨酸受体2B）参与了小鼠的骨癌疼痛。在这项研究中，我们使用骨癌疼痛的小鼠模型研究roscovitine是否可以通过调节脊髓中NR2B mRNA的表达水平来减轻骨癌疼痛。将C3H / HeJ小鼠的骨肉瘤细胞接种到右股骨的髓内腔中，以诱导正在进行的骨癌疼痛行为。术后第14天，接种骨肉瘤细胞可显着增强机械性异常性疼痛和热痛觉过敏，鞘内注射不同剂量的roscovitine可减轻这种疼痛。与疼痛行为的变化相关，我们研究中的RT-PCR实验表明，手术后脊髓中NR2B mRNA的表达明显增加，而鞘内注射roscovitine可以减轻这种情况。这些结果表明，roscovitine可能是治疗骨癌疼痛的有用辅助疗法，脊髓中的NR2B可能参与了这一作用。

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