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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption
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Gene expression in the ventral tegmental area of 5 pairs of rat lines selectively bred for high or low ethanol consumption

机译：高或低乙醇消耗选择性繁殖的5对大鼠系腹盖区基因表达

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摘要

The objective of this study was to determine if there are common innate differences in gene expression or gene pathways in the ventral tegmental area (VTA) among 5 different pairs of rat lines selectively bred for high (HEC) or low (LEC) ethanol consumption: (a) alcohol-preferring (P) vs. alcohol-non-preferring (NP) rats; (b) high-alcohol-drinking (HAD) vs. low-alcohol-drinking (LAD) rats (replicate line pairs 1 and 2); (c) ALKO alcohol (AA) vs. nonalcohol (ANA) rats; and (d) Sardinian alcohol-preferring (sP) vs. alcohol-nonpreferring (sNP) rats. Microarray analysis revealed between 370 and 1340 unique named genes that significantly differed in expression between the individual line-pairs. Analysis using Gene Ontology (GO) and Ingenuity Pathways information indicated significant categories and networks in common for up to 3 line-pairs, but not for all 5 line-pairs; moreover, there were few genes in common in these categories and networks. ANOVA of the combined data for the 5 line-pairs indicated 1295 significant (p < 0.01) differences in expression of named genes. Although no individual named gene was significant across all 5 line-pairs, there were 22 genes that overlapped in the same direction in 3 or 4 of the line-pairs. Overall, the findings suggest that (a) some biological categories or networks may be in common for subsets of line-pairs; and (b) regulation of different genes and/or combinations of multiple biological systems (e.g., transcription, synaptic function, intracellular signaling and protection against oxidative stress) within the VTA (possibly involving dopamine and glutamate) may be contributing to the disparate alcohol drinking behaviors of these line-pairs.
机译：这项研究的目的是确定在选择食用高乙醇（HEC）或低乙醇（LEC）的5对不同大鼠对中，腹侧被盖区（VTA）的基因表达或基因途径是否存在常见的先天差异： （a）酒精偏爱（P）与非酒精偏爱（NP）大鼠; （b）高酒精饮料（HAD）与低酒精饮料（LAD）大鼠（重复第1行和第2行）; （c）ALKO酒精（AA）与非酒精（ANA）大鼠; （d）撒丁岛酒精偏爱（sP）与非酒精偏爱（sNP）大鼠。微阵列分析揭示了在370个和1340个之间的独特命名基因，这些基因在各个线对之间的表达差异显着。使用基因本体论（GO）和“创造力途径”信息进行的分析表明，多达3个线对的重要类别和网络共有，但对所有5个线对却不是。此外，在这些类别和网络中几乎没有共同的基因。 5个线对的组合数据的方差分析表明命名基因的表达存在1295个显着（p <0.01）差异。尽管没有一个单独的命名基因在所有5个线对中均具有显着性，但在3个或4个线对中有22个基因在同一方向上重叠。总体而言，研究结果表明：（a）某些线对的子集可能具有某些生物学类别或网络; （b）调节VTA中的不同基因和/或多种生物系统（例如，转录，突触功能，细胞内信号转导和抗氧化应激的保护）的组合（可能涉及多巴胺和谷氨酸）可能是导致饮酒差异的原因这些线对的行为。

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