Alcohol binge-drinking, especially among adolescents and young adults, is a serious public health concern. The present study examined ethanol binge-like drinking by peri-adolescent [postnatal days (PNDs 30-72)] and adult (PNDs 90-132) alcohol-preferring (P) rats with a drinking-in-the-dark-multiple-scheduled-access (DID-MSA) procedure used by our laboratory. Male and female P rats were provided concurrent access to 15% and 30% ethanol for three 1-h sessions across the dark cycle 5 days/week. For the 1st week, adolescent and adult female P rats consumed 3.4 and 1.6g/kg of ethanol, respectively, during the 1st hour of access, whereas for male rats the values were 3.5 and 1.1g/kg of ethanol, respectively. Adult intakes increased to ~2.0 g/kg/h and adolescent intakes decreased to ~2.5 g/kg/h across the 6 weeks of ethanol access. The daily ethanol intake of adult DID-MSA rats approximated or modestly exceeded that seen in continuous access (CA) rats or the selection criterion for P rats (>/=5 g/kg/day). However, in general, the daily ethanol intake of DID-MSA peri-adolescent rats significantly exceeded that of their CA counterparts. BELs were assessed at 15-min intervals across the 3rd hour of access during the 4th week. Ethanol intake was 1.7 g/kg vs. 2.7 g/kg and BELs were 57 mg% vs. 100mg% at 15- and 60-min, respectively. Intoxication induced by DID-MSA in female P rats was assessed during the 1st vs. 4th week of ethanol access. Level of impairment did not differ between the 2 weeks (106 vs. 97 s latency to fall, 120 s criterion) and was significant (vs. naive controls) only during the 4th week. Overall, these findings support the use of the DID-MSA procedure in rats, and underscore the presence of age- and sex-dependent effects mediating ethanol binge-like drinking in P rats.
展开▼
机译：酗酒，尤其是在青少年中，是严重的公共卫生问题。本研究检查了青少年期[产后天数（PNDs 30-72）]和成年（PNDs 90-132）嗜酒精（P）大鼠在黑暗中多次饮酒的酒精暴饮状饮酒。我们实验室使用的计划访问（DID-MSA）程序。在5天/周的黑暗周期中，雄性和雌性P大鼠在黑暗周期中连续三个1小时提供了15％和30％乙醇的同时使用。在进入第1周的第1周，青春期和成年雌性P大鼠分别摄入3.4和1.6g / kg乙醇，而雄性大鼠分别为3.5和1.1g / kg乙醇。在摄入酒精的6周内，成人摄入量增加到〜2.0 g / kg / h，青少年摄入量减少到〜2.5 g / kg / h。成年DID-MSA大鼠的每日乙醇摄入量近似或适度超过了连续访问（CA）大鼠或P大鼠的选择标准（> / = 5 g / kg /天）。但是，总的来说，DID-MSA青春期大鼠的每日乙醇摄入量大大超过了其CA对应者。在第4周的访问的第3小时内，以15分钟为间隔评估BEL。在15分钟和60分钟时，乙醇的摄入量分别为1.7 g / kg和2.7 g / kg，BELs分别为57 mg％和100mg％。在摄入乙醇的第1周至第4周，评估了DID-MSA对雌性P大鼠的中毒情况。 2周之间的损伤程度无差异（跌落潜伏期106 vs. 97 s，标准为120 s），仅在第4周才显着（相对于幼稚对照组）。总体而言，这些发现支持在大鼠中使用DID-MSA程序，并强调了介导P大鼠中酒精暴饮般饮酒的年龄和性别依赖性效应的存在。
展开▼
