High-Speed Extraction and HPLC Fingerprinting of Medicinal Plants - II. Application to Harman Alkaloids of Genus Passiflora

Ehab A. Abouralshed; John Vanderplank; Ikhlas A. Khan

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High-Speed Extraction and HPLC Fingerprinting of Medicinal Plants - II. Application to Harman Alkaloids of Genus Passiflora

机译：药用植物的高速萃取和HPLC指纹图谱-II。西番莲属哈曼生物碱的应用

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Although the presence of harman alkaloids in the genus Passiflora has been established in a few species, their presence in the medicinally significant P. incarnata (passion flower) is still debatable. Powdered samples from 91 Passiflora species were subjected to accelerated speed extraction and analyzed for their content of five harman alkaloids by reversed-phase HPLC with photodiode array detection. The utilized analytical method was capable of detecting harman alkaloids in more than 50% of the analyzed samples at ppm levels. A library of HPLC chromatograms has also been generated as a tool for fingerprinting and authentication of the studied Passiflora species.

机译：尽管西番莲属中存在哈曼生物碱存在于少数物种中，但它们仍存在于具有药用价值的P. incarnata（西番莲）中仍有争议。对来自91个西番莲属的粉末状样品进行加速萃取，并通过反相HPLC和光电二极管阵列检测分析五种哈曼生物碱的含量。利用的分析方法能够以ppm的浓度检测到超过50％的分析样品中的哈曼生物碱。 HPLC色谱图谱库也已生成，可作为指纹图谱和鉴定所研究西番莲物种的工具。

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《Pharmaceutical Biology》 |2003年第2期|共7页
作者
Ehab A. Abouralshed; John Vanderplank; Ikhlas A. Khan;
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正文语种 eng
中图分类药学;
关键词
Passion flower; passion fruit; Passifloraceae; harman alkaloids; accelerated extraction; HPLC.;

机译：百香花;百香果;西番莲科;哈曼生物碱;加速提取;HPLC。;

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1. High-Speed Extraction and HPLC Fingerprinting of Medicinal Plants - II. Application to Harman Alkaloids of Genus Passiflora [J] . Ehab A. Abouralshed, John Vanderplank, Ikhlas A. Khan Pharmaceutical Biology . 2003,第2期

机译：药用植物的高速萃取和HPLC指纹图谱-II。西番莲属哈曼生物碱的应用
2. Chemical fingerprint and simultaneous determination of alkaloids and flavonoids in aerial parts of genus Peganum indigenous to China based on HPLC-UV: application of analysis on secondary metabolites accumulation [J] . Wen Fangfang, Cheng Xuemei, Liu Wei, Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences . 2014,第12期

机译：基于HPLC-UV的中国本地Pe属植物地上部分化学指纹图谱和同时测定生物碱和类黄酮：次生代谢物积累分析的应用
3. Discrimination of three medicinal materials from the Citrus genus by HPLC fingerprint coupled with two complementary software. [J] . Qin XM, Dai YT, Zhang LZ, Phytochemical Analysis . 2009,第4期

机译：HPLC指纹图谱结合两个互补软件，可从柑橘属中区分出三种药用材料。
4. Determination of amentoflavone in Selaginella medicinal herbs from different areas and study on HPLC fingerprint characteristics of Selaginella plants [C] . XUE Feng-Ning, LIU Hong-Xia, ZHANG Guo-Gang Proceedings of the 6th CCTCNM-KSP-JSP joint symposium on pharmacognosy . 2011

机译：不同地区卷柏药材中黄酮含量的测定及卷柏植物的HPLC指纹图谱研究
5. Part I. Evaluation of silver as a reductive amperometric detector electrode for HPLC and FIA. Part II. Evaluation of supercritical fluid extraction (SFE) as a sample preparation technique. Part III. Development and validation of HPLC methods using supercritical fluid extraction and reversed-phase HPLC with electrochemical detection (LCEC). [D] . Schneiderman, Martin Alan. 1990

机译：第一部分：银作为HPLC和FIA的还原型安培检测器电极的评估。第二部分评价超临界流体萃取（SFE）作为样品制备技术。第三部分使用超临界流体萃取和带电化学检测的反相HPLC（LCEC）的HPLC方法的开发和验证。
6. The acquisition of alkaloids from plant materials by a combination of ion-pair extraction and preparative ion-pair HPLC using sodium perchlorate. [O] . Masataka MORIYASU, Momoyo ICHIMARU, Yumi NISHIYAMA, 1993

机译：使用高氯酸钠的离子对萃取和制备型离子对HPLC的组合从植物材料中获取生物碱。

High-Speed Extraction and HPLC Fingerprinting of Medicinal Plants - II. Application to Harman Alkaloids of Genus Passiflora

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