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Antiviral effects of the constituents derived from Chinese herb medicines on infectious bursal disease virus

机译：中草药成分对传染性法氏囊病病毒的抗病毒作用

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Context: The prevalence of infectious bursal disease has brought about enormous financial losses to the world poultry industry. Chinese herb medicines can provide valuable materials for discovery and development of new drugs.Objective: To screen constituents derived from Chinese herb medicines for their antiviral activity against infectious bursal disease virus (IBDV) in vitro.Materials and methods: Twenty constituents derived from Chinese herb medicines and B87 strain of IBDV were used. The 50% cytotoxic concentration (CC_50) and 50% effective concentration (EC_50) were determined by visualization of cytopathologic effect (CPE) and 3-(4,5-dimethyithia-zol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) test on chicken embryo fibroblast. Selectivity index (SI) and inhibition ratio (%l) were calculated from the data obtained from the MTT test. Results: Antiviral assays showed dipotassium glycyrrhizinate and ligustrazine hydrochloride among the 20 constituents tested exhibited significant inhibitory activity against IBDV in a dose-dependent manner. EC_50 of dipotassium glycyrrhizinate and ligustrazine hydrochloride were 663.2 +-268.4 and 92.52 +- 21.13 ng/mL, and SI were >4.52 and >21.62, respectively. The time-of-addition and virucidal assay indicated that anti-IBDV activity of the two constituents could be due to their inhibiting virus replication and/or inactivating virus directly. The inhibition of virus attachment was not observed in the adsorption inhibition assay. Dipotassium glycyrrhizinate and ligustrazine hydrochloride exhibited more than 70% and 80% inhibition of IBDV, respectively, at the maximum safe concentration.Discussion and conclusion: We believe that dipotassium glycyrrhizinate and ligustrazine hydrochloride can be used to develop a new anti-IBDV compound, and it is worth applying the constituents in clinical practice.
机译：背景：传染性法氏囊病的流行给世界家禽业带来了巨大的经济损失。中草药可以为新药的发现和开发提供有价值的材料。目的：筛选中草药衍生的成分在体外对传染性法氏囊病病毒（IBDV）的抗病毒活性。材料和方法：20种中草药衍生的成分使用药物和IBDV的B87株。通过可视化细胞病理学效应（CPE）和3-（4,5-dimethyithia-zol-2-yl）-2,5-diphenyltetrazoliumbromide（MTT）来确定50％细胞毒性浓度（CC_50）和50％有效浓度（EC_50） ）在鸡胚成纤维细胞上进行测试。从MTT测试获得的数据计算出选择性指数（SI）和抑制率（％1）。结果：抗病毒测定显示，被测的20种成分中的甘草酸双钾盐和盐酸川gust嗪对IBDV表现出显着的抑制活性，且呈剂量依赖性。甘草次酸二钾和盐酸川gust嗪的EC_50为663.2 + -268.4和92.52 +-21.13 ng / mL，SI分别为> 4.52和> 21.62。添加时间和杀病毒试验表明这两种成分的抗IBDV活性可能是由于它们直接抑制病毒复制和/或灭活病毒所致。在吸附抑制试验中未观察到病毒附着的抑制。在最大安全浓度下，甘草酸二钾和盐酸川gust嗪分别表现出超过70％和80％的抑制作用。讨论和结论：我们认为，甘草酸二钾和盐酸川gust嗪可以用于开发新的抗IBDV化合物，并且值得在临床实践中应用这些成分。

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