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首页> 外文期刊>Philosophical transactions of the Royal Society. Mathematical, physical, and engineering sciences >Exploring the in meso crystallization mechanism by characterizing the lipid mesophase microenvironment during the growth of single transmembrane alpha-helical peptide crystals
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Exploring the in meso crystallization mechanism by characterizing the lipid mesophase microenvironment during the growth of single transmembrane alpha-helical peptide crystals

机译：通过表征单个跨膜α-螺旋肽晶体生长过程中的脂质中间相微环境，探索介观结晶机理

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摘要

The proposed mechanism for in meso crystallization of transmembrane proteins suggests that a protein or peptide is initially uniformly dispersed in the lipid self-assembly cubic phase but that crystals grow from a local lamellar phase, which acts as a conduit between the crystal and the bulk cubic phase. However, there is very limited experimental evidence for this theory. We have developed protocols to investigate the lipid mesophase microenvironment during crystal growth using standard procedures readily available in crystallography laboratories. This technique was used to characterize the microenvironment during crystal growth of the DAP12-TM peptide using synchrotron small angle X-ray scattering (SAXS) with a micro-sized X-ray beam. Crystal growth was found to occur from the gyroid cubic mesophase. For one in four crystals, a highly oriented local lamellar phase was observed, providing supporting evidence for the proposed mechanism for in meso crystallization. A new observation of this study was that we can differentiate diffraction peaks from crystals grown in meso, from peaks originating from the surrounding lipid matrix, potentially opening up the possibility of high-throughput SAXS analysis of in meso grown crystals.
机译：跨膜蛋白介观结晶的拟议机制表明，蛋白质或肽最初均匀地分散在脂质自组装立方相中，但晶体从局部层状相生长，该层状相充当了晶体与大块立方之间的通道。相。但是，该理论的实验证据非常有限。我们已经开发了使用晶体学实验室中容易获得的标准程序研究晶体生长过程中脂质中间相微环境的方案。使用同步加速器小角度X射线散射（SAXS）和微型X射线束，该技术用于表征DAP12-TM肽晶体生长过程中的微环境。发现晶体生长是从回旋中间相产生的。对于四分之一的晶体，观察到高度取向的局部层状相，为提出的介观结晶机理提供了支持证据。这项研究的一项新观察结果是，我们可以区分中观生长晶体的衍射峰和周围脂质基质产生的峰，从而有可能对中观生长晶体进行高通量SAXS分析。

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