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首页> 外文期刊>Physics in medicine and biology. >Quantitative spatially resolved measurement of tissue chromophore concentrations using photoacoustic spectroscopy: application to the measurement of blood oxygenation and haemoglobin concentration
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Quantitative spatially resolved measurement of tissue chromophore concentrations using photoacoustic spectroscopy: application to the measurement of blood oxygenation and haemoglobin concentration

机译：使用光声光谱法对组织生色团浓度进行空间分辨定量测量：在血液氧合和血红蛋白浓度测量中的应用

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摘要

A new approach based on pulsed photoacoustic spectroscopy for non-invasively quantifying tissue chromophore concentrations with high spatial resolution has been developed. The technique is applicable to the quantification of tissue chromophores such as oxyhaemoglobin ( HbO(2)) and deoxyhaemoglobin ( HHb) for the measurement of physiological parameters such as blood oxygen saturation ( SO2) and total haemoglobin concentration. It can also be used to quantify the local accumulation of targeted contrast agents used in photoacoustic molecular imaging. The technique employs a model-based inversion scheme to recover the chromophore concentrations from photoacoustic measurements. This comprises a numerical forward model of the detected time-dependent photoacoustic signal that incorporates a multiwavelength diffusion-based finite element light propagation model to describe the light transport and a time-domain acoustic model to describe the generation, propagation and detection of the photoacoustic wave. The forward model is then inverted by iteratively fitting it to measurements of photoacoustic signals acquired at different wavelengths to recover the chromophore concentrations. To validate this approach, photoacoustic signals were generated in a tissue phantom using nanosecond laser pulses between 740 nm and 1040 nm. The tissue phantom comprised a suspension of intralipid, blood and a near-infrared dye in which three tubes were immersed. Blood at physiological haemoglobin concentrations and oxygen saturation levels ranging from 2% to 100% was circulated through the tubes. The signal amplitude from different temporal sections of the detected photoacoustic waveforms was plotted as a function of wavelength and the forward model fitted to these data to recover the concentrations of HbO(2) and HHb, total haemoglobin concentration and SO2. The performance was found to compare favourably to that of a laboratory CO-oximeterwith measurement resolutions of +/- 3.8 g l(-1) ( +/- 58 mu M) and +/- 4.4 g l(-1) ( +/- 68 mu M) for the HbO(2) and HHb concentrations respectively and +/- 4% for SO2 with an accuracy in the latter in the range -6%-+7%.
机译：已经开发了一种基于脉冲光声光谱技术的新方法，该方法可非侵入性地以高空间分辨率量化组织发色团的浓度。该技术适用于组织生色团的定量，例如氧合血红蛋白（HbO（2））和脱氧血红蛋白（HHb），用于测量生理参数，例如血氧饱和度（SO2）和总血红蛋白浓度。它也可以用于量化光声分子成像中使用的目标造影剂的局部累积。该技术采用基于模型的反演方案来从光声测量中恢复生色团浓度。这包括检测到的随时间变化的光声信号的数值正向模型，该模型包含基于多波长扩散的有限元光传播模型（用于描述光传输）和时域声学模型（用于描述光声波的生成，传播和检测） 。然后，通过将迭代模型迭代拟合到在不同波长下获取的光声信号的测量值，以恢复生色团浓度，从而反转前向模型。为了验证该方法，使用740 nm至1040 nm之间的纳秒激光脉冲在组织体模中生成了光声信号。组织体模包括脂质内，血液和近红外染料的悬浮液，其中将三个管浸入其中。使生理血红蛋白浓度和氧饱和度范围从2％到100％的血液通过试管循环。将来自检测到的光声波形的不同时间部分的信号幅度绘制为波长的函数，并将正向模型拟合到这些数据以恢复HbO（2）和HHb的浓度，总血红蛋白浓度和SO2。发现该性能可与实验室CO血氧仪相比，测量分辨率为+/- 3.8 gl（-1）（+/- 58μM）和+/- 4.4 gl（-1）（+/- 68 μM）分别用于HbO（2）和HHb浓度，+ /-2％用于SO2，后者的精度在6％-+ 7％范围内。

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