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首页> 外文期刊>Biomaterials >Synaptic plasticity in micropatterned neuronal networks.
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Synaptic plasticity in micropatterned neuronal networks.

机译：微模式神经元网络中的突触可塑性。

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摘要

Synaptic plasticity is thought to be of central importance for information processing by the nervous system. Additionally, specific neuronal connectivity patterns in the brain are implicated to play a role in the perception, processing and storage of incoming signals. Experimental control over connectivity within functional neuronal networks is therefore a promising approach in research on signal transduction and processing by the nervous system. A cell culture system is presented that allows experimental determination of neuronal connectivity patterns in an in vitro network. Rat embryonic cortical neurons were grown on patterns of extracellular matrix proteins applied to polystyrene substrates by microcontact printing. Cells comply well with the pattern and form synaptic connections along the experimentally defined pathways. Chemical synapses identified by double patch-clamp measurement showed paired pulse depression as well as frequency-dependent depression in response to trains of stimuli. This typeof short-term plasticity has similarly been reported by others in brain slices. Thus, the system reproduces features central for neuronal information processing while the architecture of the network is experimentally manipulable. The ability to tailor the geometry of functional neuronal networks offers a valuable tool both for fundamental questions in neuroscientific research and a wide range of biotechnological applications.
机译：突触可塑性被认为对于神经系统的信息处理至关重要。另外，暗示大脑中特定的神经元连接模式在传入信号的感知，处理和存储中发挥作用。因此，对功能神经元网络内的连接性进行实验控制是神经系统信号转导和处理研究中的一种有前途的方法。提出了一种细胞培养系统，其允许在体外网络中实验确定神经元连接模式。通过微接触印刷法将大鼠胚胎皮质神经元生长在应用于聚苯乙烯底物的细胞外基质蛋白上。细胞很好地符合模式并沿着实验确定的途径形成突触连接。通过双膜片钳测量确定的化学突触显示成对的脉冲抑制以及响应于刺激序列的频率依赖性抑制。他人在大脑切片中也报告了这种类型的短期可塑性。因此，该系统再现了神经元信息处理中心的特征，而网络的体系结构可通过实验操作。定制功能神经元网络的几何形状的能力为神经科学研究中的基本问题和广泛的生物技术应用提供了宝贵的工具。

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