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首页> 外文期刊>Biomaterials >Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures.
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Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures.

机译：由纤维蛋白原和聚乙二醇制成的生物合成水凝胶支架，用于3D细胞培养。

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摘要

Tissue engineering scaffolds are fabricated from either biological materials, which provide biofunctional signals and interact well with cells, or from synthetic polymers, which provide precise control over their structural properties. We describe a biosynthetic hybrid scaffold comprised of a fibrinogen backbone and crosslinked with difunctional polyethylene glycol (PEG) side chains. Denatured fibrinogen fragments are PEGylated with PEG-diacrylates, mixed with photoinitiator and exposed to UV light to form a hydrogel material in the presence of a cell suspension. This unique hydrogel material provides a distinct advantage over other scaffold materials because its mechanical properties are highly malleable while the biological functionality is maintained by the backbone of the polymeric network. The elastic modulus of the PEG-fibrinogen hydrogel is dependent on the molecular weight of the PEG constituent and proportional to the percent polymeric composition. The biological domains in the fibrinogen backbone provide attachment motifs for endothelial cell and smooth muscle cell adhesion as well as proteolytic sensitivity for biodegradation. Smooth muscle cells demonstrate the ability to proteolytically penetrate through the hydrogel material and form interconnecting networks of cells. Our efforts to develop novel biodegradable scaffolds for cultivating cells in a 3D environment are beneficial for tissue regeneration therapies.
机译：组织工程支架可以由提供生物功能信号并与细胞良好相互作用的生物材料制成，也可以由提供对其结构特性的精确控制的合成聚合物制成。我们描述了一种由纤维蛋白原骨架组成的生物合成混合支架，并与双功能聚乙二醇（PEG）侧链交联。将变性的纤维蛋白原片段用PEG-二丙烯酸酯PEG化，与光引发剂混合，并在细胞悬液存在的情况下暴露于紫外线下以形成水凝胶材料。这种独特的水凝胶材料提供了优于其他支架材料的独特优势，因为它的机械性能具有很高的延展性，而生物学功能是由聚合物网络的主链保持的。 PEG-纤维蛋白原水凝胶的弹性模量取决于PEG成分的分子量，并与聚合物组成的百分比成比例。纤维蛋白原主链中的生物学结构域提供了内皮细胞和平滑肌细胞粘附的附着基序，以及对生物降解的蛋白水解敏感性。平滑肌细胞表现出蛋白水解能力穿透水凝胶材料并形成相互连接的细胞网络的能力。我们开发用于在3D环境中培养细胞的新型可生物降解支架的努力对于组织再生疗法是有益的。

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